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李平丽.牛磺酸对宫内生长受限孕鼠生长轴激素与基因的影响[J].中国实验动物学报,2020,28(3):345~350.
牛磺酸对宫内生长受限孕鼠生长轴激素与基因的影响
Effects of taurine on growth axis hormones and genes in pregnant rats with intrauterine growth restriction
投稿时间:2019-10-27  
DOI:10. 3969 / j.issn.1005-4847. 2020. 03. 008
中文关键词:  牛磺酸  生长受限孕鼠  胰岛素样生长因子 1
英文关键词:taurine  growth-restricted pregnant rats  insulin-like growth factor 1
基金项目:
作者单位E-mail
李平丽 沈阳农业大学畜牧兽医学院,沈阳 110866 1244822747@ qq.com 
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中文摘要:
      目的 观察牛磺酸对宫内生长受限孕鼠血液激素与 IGF 基因表达的影响。 方法 取健康 SD 系大鼠雌雄 2 ∶1合笼饲养,按妊娠顺序随机抽取孕鼠分为正常饲料组(C)、低蛋白饲料组(M)、低蛋白饲料+牛磺酸组 (MT),正常饲料+牛磺酸组(T)。 每组孕鼠各 10 只,M、MT 组孕期采取低蛋白饲料喂养造模,孕第 12 天,C 和 M 组孕鼠灌胃饮用水,MT 和 T 组灌胃牛磺酸 300 mg / (kg·d)。 每周检测孕鼠体重增长情况,孕 21 d 取样,记录胎鼠体重、数量、畸胎数;Elisa 方法检测孕鼠血清生长激素(GH)、胰岛素生长因子 1(IGF-1)、胰岛素(INS)、甲状腺素(T3 和 T4)水平,荧光定量 PCR 方法检测孕鼠肝生长激素受体(GHR)、胰岛素生长因子 1( IGF-1)、胰岛素生长因子结合蛋白 1(IGFBP-1)和胰岛素生长因子结合蛋白 3(IGFBP-3)的 mRNA 表达量。 结果 添加牛磺酸组胎鼠体重、数量和畸胎明显改善,孕鼠体重增加显着(P < 0. 05),血液 INS 和 T4 增加显着(P < 0. 05),肝 IGF-1、IGFBP-3 mRNA 表达升高显着(P < 0. 05),IGFBP-1 mRNA 显着降低(P < 0. 01)。 结论 添加牛磺酸能够改善低蛋白孕鼠甲状腺功 能、GH/ IGF-1 轴紊乱,这可能在缓解胎儿子宫生长受限中具有重要的作用。
英文摘要:
      Objective To observe the effects of taurine on blood hormones and hepatic insulin growth factor (IGF) gene expression in intrauterine growth-restricted pregnant rats. Methods Healthy SD female and male rats were housed 1 ∶2 in cages and pregnant rats were randomly selected into the blank control group (C), model group (M), model + taurine group (MT), and taurine group (T). In the M and MT groups, 10 pregnant rats were fed a low-protein diet. Day 12 of pregnancy, C, and M were provided drinking water, and MT and T groups received taurine 300 mg / (kg·d). Weekly monitoring of weight gain in pregnant rats. At day 21 of gestation, baseline characteristics of fetuses were determined. Serum growth hormone (GH), insulin growth factor 1 (IGF-1), insulin (INS), and thyroxine (T3 and T4) levels were detected by ELISA. Fluorescent quantitative PCR was used to detect the hepatic expression levels of growth hormone receptor (GHR), insulin growth factoR-1 ( IGF-1), insulin growth factor binding protein-1 ( IGFBP-1) and insulin growth factor binding protein-3 ( IGFP-3) mRNA in pregnant rats. Results Taurine markedly improved the baseline characteristics, compared with the M group. Pregnant rats in the MT group had significantly increased (P < 0. 05) weights, blood INS and T4 levels, and liver IGF-1 and IGFBP-3 mRNA expression, whereas IGFBP-1 mRNA expression was significantly reduced (P < 0. 01). Conclusions The addition of taurine can improve the thyroid function and GH/ IGF-1 axis disorder in low- protein diet pregnant rats, and may play an important role in alleviating fetal growth restriction.
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