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白梦天.不同条件下建立过敏性结膜炎小鼠模型的比较研究[J].中国实验动物学报,2020,28(3):330~337.
不同条件下建立过敏性结膜炎小鼠模型的比较研究
Comparison of mouse models of allergic conjunctivitis under different conditions
投稿时间:2019-11-18  
DOI:10. 3969 / j.issn.1005-4847. 2020. 03. 006
中文关键词:  过敏性结膜炎  白介素 17  屋尘螨  卵清蛋白  豚草花粉
英文关键词:allergic conjunctivitis  interleukin-17  house dust mites  ovalbumin  ragweed pollen
基金项目:
作者单位E-mail
白梦天 1.昆明医科大学第四附属医院眼科,昆明 650032
2. 云南省眼科研究所,昆明 650032
3. 云南省第二人民 医院眼科,昆明 650032
4. 姚克专家工作站,昆明 650032 
308347673@ qq.com 
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中文摘要:
      目的 分别在不同抗原、不同激发途径、不同小鼠周龄条件下,建立过敏性结膜炎小鼠模型,通过比较免疫应答状态,筛选最佳致敏条件,为过敏性结膜炎小鼠动物模型的建立提供新的思路和方法。 方法 SPF 级 Balb / c 小鼠 65 只,在不同抗原(卵清蛋白、豚草花粉、屋尘螨),不同激发途径(灌胃、雾化吸入、皮下注射),不同小 鼠周龄(1 周龄、2 周龄、3 周龄、8 周龄)条件下构建过敏性结膜炎小鼠动物模型。RT-qPCR 检测小鼠结膜组织中 CCL5 mRNA、IL-17 mRNA 相对表达水平,流式细胞术检测脾脏单细胞悬液 IL-17 占 CD4+ T 细胞百分比。 结果 同阴性对照组比较,卵清蛋白组、屋尘螨组、豚草花粉组 IL-17 百分比、CCL5 mRNA 及 IL-17 mRNA 表达均上升,其中, 以豚草花粉组上升的最为显著。同阴性对照组比较,皮下注射组、灌胃组、雾化吸入组、阳性对照组 IL-17 百分比、 IL-17 mRNA表达均上升,CCL5 mRNA 在灌胃组中的表达与阴性对照组接近,皮下注射组的表达水平更接近阳性对照组。 1 周龄、2 周龄组小鼠 IL-17 百分比、CCL5 mRNA、IL-17 mRNA 表达水平较为接近,但显著低于 3 周龄、8 周龄组小鼠。 结论 豚草花粉在构建过敏性结膜炎小鼠动物模型中具有更好的致敏性。皮下注射法激发抗原在过 敏性结膜炎小鼠动物模型构造中可取得较满意效果,尽管未见明显眼部症状及体征,但全身和局部均呈致敏状态。 3 周龄 Balb / c 小鼠免疫应答反应已较为接近成年 Balb / c 小鼠。
英文摘要:
      Objective To establish a mouse model of allergic conjunctivitis by comparison of different antigens, routes of administration, and ages of mice. We compared their immune response status and screened for optimal sensitization conditions to provide new ideas and method to establish mouse models of allergic conjunctivitis. Methods Sixty-five SPF Balb / c mice were selected to establish allergic conjunctivitis models using different antigens ( ovalbumin, ragweed pollen, house dust mite ), administration routes ( gastric administration, aerosol inhalation, subcutaneous injection) and ages ( 1, 2, 3 and 8 weeks). Results The expression levels of interleukin-17 ( IL-17) and C-C motif chemokine ligand 5 ( CCL5) mRNA were increased in the ovalbumin, house dust mite and ragweed pollen groups, especially the later, compared with the negative control group. Compared with the negative control group, the expression of IL-17 mRNA was increased in the subcutaneous injection, gavage, inhalation and positive control groups. The expression of CCL5 mRNA in the gavage group was close to that of the negative control group, while its expression level in the subcutaneous injection group was closer to that of the positive control group. Expression levels of CCL5 and IL-17 mRNA were similar in 1 - and 2-week-old mice, but significantly lower than those in 3 - and 8-week-old mice. Conclusions Ragweed pollen provided better sensitization in the preparation of an allergic conjunctivitis mouse model. Subcutaneous injection of antigen achieved satisfactory result in the allergic conjunctivitis mouse model. Although there were no obvious eye symptoms, the whole body and local areas were sensitized. The immune response of 3-week-old Balb / c mice was close to that of adult Balb / c mice.
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