Objective To explore the effects of microRNAs ( miRNAs ) on proliferation, migration and angiogenesis in a miniature pig model of myocardial infarction (MI). The miRNAs differently expressed between MI and control pigs were selected, in particular those potentially targeting the ANGPT-2 gene ( an important growth factor during angiogenesis), and their effects on proliferation, migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) were investigated. Methods Bioinformatics software was used to predict the differentially expressed miRNAs targeting ANGPT-2 in the MI and control groups. The effects of miRNAs on the proliferation, migration and angiogenesis of HUVECS were detected by EdU staining proliferation, transwell chamber and in vitro experimental method, respectively. Results miR-144, miR-21-3p, miR-142-5p and miR-27b-3p were found to potentially target ANGPT-2 and were significantly differently expressed between the MI and control groups. In HUVECs, miR-144 had no significant effect on proliferation, but significantly inhibited migration and vessel formation; miR-21-3p inhibited proliferation and migration, but promoted vessel formation; miR-142-5p and miR-27b-3p inhibited proliferation, migration and vessel formation. Of these four miRNAs, miR-142-5p and miR-27b-3p significantly inhibited the expression of ANGPT-2 mRNA. Conclusions After MI, miR-142-5p and miR-27b-3p may inhibit angiogenesis by suppressing the proliferation, migration and vessel formation of vascular endothelial cells, by targeting the 3′ UTR of ANGPT-2 and down-regulating ANGPT-2 mRNA expression.