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杨颖雯,周瑾,黄菲.不同年龄段小鼠抑郁及焦虑样行为的比较[J].中国实验动物学报,2020,28(2):0.
不同年龄段小鼠抑郁及焦虑样行为的比较
Comparison of depression-like and anxiety-like behaviors in mice of different ages
投稿时间:2019-09-10  
DOI:10. 3969 / j.issn.1005-4847. 2020. 02. 015
中文关键词:  年龄段  抑郁样行为、焦虑样行为  5-羟色胺
英文关键词:age  depression-like behavior  anxiety-like behavior  5-HT
基金项目:
作者单位E-mail
杨颖雯 上海中医药大学中药研究所, 上海中药标准化研究中心,上海 201203 hellokittyyyw@ 163.com 
周瑾 上海中医药大学中药研究所, 上海中药标准化研究中心,上海 201203  
黄菲 上海中医药大学中药研究所, 上海中药标准化研究中心,上海 201203 Fei_H@ hotmail.com 
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中文摘要:
      目的 观察和比较不同年龄段实验小鼠的抑郁及焦虑样行为。 方法 将 27 只雄性 C57BL/ 6 小鼠 按年龄分为青年组(3 月龄, n = 9)、中年组(10 月龄, n = 9)、老年组(18 月龄, n = 9),分别进行悬尾测试、强迫游泳测试、高架十字迷宫测试、开放旷场测试和糖水偏好测试, 观察小鼠抑郁及焦虑样行为,并用液质联用(LC-MS / MS) 法检测 C57BL/ 6 小鼠血清 5-羟色胺含量。 结果 中年组小鼠比老年组小鼠的悬尾不动时间长(P< 0. 05);青年 组、中年组、老年组小鼠强迫游泳不动时间显著递减,青年组小鼠游泳不动时间显著大于中年组小鼠(P< 0. 001)和 老年组小鼠(P< 0. 001),中年组小鼠游泳不动时间显著大于老年组小鼠(P< 0. 01);中年组小鼠比老年组小鼠 12 小时糖水偏好率高(P< 0. 05),青年组小鼠比老年组小鼠 12、36、48、60 小时糖水偏好率高(P< 0. 05);老年组小鼠 比中年组和青年组小鼠开臂时间长(P< 0. 05),青年组小鼠比中年组小鼠探头次数多(P< 0. 01);青年组小鼠比老 年组小鼠在旷场中的移动总距离长(P< 0. 05);老年组、中年组、青年组小鼠血清 5-HT 含量呈递减趋势,青年组小鼠血清 5-HT 显著低于中年组(P< 0. 05)和老年组(P< 0. 01)。 结论 整体而言,同一刺激下,小鼠的年龄越小,越易表现出抑郁及焦虑样行为,血清中 5-HT 水平越低。
英文摘要:
      Objective To observe and compare the depression-like and anxiety-like behaviors in mice of different ages. Methods Twenty-seven male C57BL/ 6 mice were divided into three groups according to age: young group ( 3 months, n = 9), middle-aged group (10 months, n = 9) and old group (18 months, n = 9). The tail suspension test, forced swimming test, elevated plus maze test, open field test, and sugar preference test were used to detect depression-like and anxiety-like behaviors. Liquid Chromatography with tandem mass spectrometry was used to detect 5-hydroxytryptamine (5- HT) levels in the serum of mice. Results The immobility time of middle-aged mice was longer than that of old mice in tail suspension test( P < 0. 05); the immobility time of forced swimming decreased from young to middle-aged mice ( P < 0. 001) and from middle-aged to old mice (P< 0. 01). The sucrose preference of middle-aged mice was higher than that of old mice at 12 hours (P< 0. 05); the sugar preference rate of young mice was higher than that of old mice at 12, 36, 48, and 60 hours (P< 0. 05). The time spent in the open arms by old mice was longer than that of middle-aged and young mice (P< 0. 05) and the probe number of young mice was greater than that of middle-aged mice (P< 0. 01). The total distance travelled in the open field by young mice was longer than that of old mice (P< 0. 05). The concentration of 5-HT in the serum of old, middle-aged, and young mice showed a decreasing trend. The concentration of serum 5-HT in young mice was significantly lower than that in middle-aged (P< 0. 05) and old mice (P< 0. 01). Conclusions The younger the mice, the more likely they were to exhibit depression- and anxiety-like behaviors under the same stress stimulations, and to have a lower level of 5-HT in their serum.
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