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齐国将,钟德琳,蓝海梅,郭敏,郭亚楠,彭鹏.心肌铁过载兔模型的制作及铁沉积规律的研究[J].中国实验动物学报,2020,28(2):0.
心肌铁过载兔模型的制作及铁沉积规律的研究
Establishment of a rabbit model of cardiac iron overload and its relationship with iron deposition
投稿时间:2019-10-13  
DOI:10. 3969 / j.issn.1005-4847. 2020. 02. 014
中文关键词:    铁过载  心脏铁浓度  肝铁浓度
英文关键词:rabbit  iron overload  cardiac iron concentration  liver iron concentration
基金项目:
作者单位E-mail
齐国将 广西医科大学第一附属医院放射科,南宁 530021 2643727867@ qq.com 
钟德琳 广西医科大学第一附属医院放射科,南宁 530021  
蓝海梅 广西医科大学第一附属医院放射科,南宁 530021  
郭敏 广西医科大学第一附属医院放射科,南宁 530021  
郭亚楠 广西医科大学第一附属医院放射科,南宁 530021  
彭鹏 广西医科大学第一附属医院放射科,南宁 530021 doublep@ 126.com 
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中文摘要:
      目的 探讨心肌铁沉积动物模型的制作方法及心脏、肝之间铁沉积规律的关系。 方法 选取 40 只雄性新西兰白兔随机分成实验组 32 只,对照组 8 只。 每周一实验组白兔经皮下注射右旋糖酐铁 200 mg / kg;于每 周日随机处死 2 只白兔,每间隔 1 周的周日随机处死 1 只对照组白兔;取出部分心脏、肝组织,用生理盐水反复冲洗至排尽心脏、肝组织内残留血液,置于电热恒温鼓风干燥箱(温度 65℃ )烘干至重量不再变化。烘干后的心脏、肝组织用火焰原子吸收分光光度计测量心、肝铁浓度(CIC、LIC)并将部分心、肝组织固定后行 HE 染色及普鲁士蓝染色。 结果 随着注射铁剂的增多,病理结果显示实验组家兔心肌普鲁士蓝染色铁颗粒增多,CIC 逐渐增高(范围 0. 20 ~ 2. 34 mg / g 干重,中位数 1. 24 mg / g 干重)。 CIC 与注射铁总量之间中度相关( r = 0. 698, P < 0. 05, P < 0. 001),CIC 与血清铁之间中度相关( r = 0. 415,P < 0. 05,P = 0. 022)。 CIC 与 LIC 之间高度相关( r = 0. 712,P < 0. 05, P < 0. 001)。 结论 通过皮下注射剂量为 200 mg / kg 的右旋糖酐铁,可以建立心肌铁过载兔模型,心脏、肝 之间铁沉积没有相关性。
英文摘要:
      Objective To establish an animal model of heart iron overload and investigate the relationship between iron deposition in the heart and liver. Methods Forty rabbits were randomly divided into the experimental group (n = 32) or control group ( n = 8). The experimental group was injected subcutaneously with 200 mg / kg of iron dextran every Monday. Two rabbits were randomly euthanized every Sunday. The heart and liver tissues were removed, rinsed with normal saline repeatedly, and placed in an oven at 65℃ to dry until the weight was unchanged. A flame atomic absorption spectrophotometer was used to measure the iron concentration of hearts and livers ( CIC, LIC). Hematoxylin and eosin staining and Prussian blue staining were performed. Results With the increase of iron injection, pathological result showed that Prussian blue staining of iron particles increased in the experimental group, and that the CIC increased gradually (0. 20-2. 34 mg / g dry weight, median 1. 24 mg / g dry weight). CIC was moderately correlated with total injected iron (r = 0. 698, P < 0. 05,P< 0. 001), and CIC was moderately correlated with serum iron (r = 0. 415, P < 0. 05, P = 0. 022). CIC was highly correlated with LIC (r= 0. 712, P< 0. 05, P< 0. 001). Conclusions A rabbit model of cardiac iron overload was established by the subcutaneous injection of dextran iron at a dose of 200 mg / kg, and there was no correlation between heart and liver iron deposition.
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