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张迪,夏艺,范丽,刘士远,管宇.慢性阻塞性肺疾病大鼠模型的建立与评价[J].中国实验动物学报,2020,28(2):0.
慢性阻塞性肺疾病大鼠模型的建立与评价
Establishment and evaluation of rat models of chronic obstructive pulmonary disease
投稿时间:2019-08-17  
DOI:10. 3969 / j.issn.1005-4847. 2020. 02. 012
中文关键词:  动物模型  肺疾病,慢性阻塞性  体层摄影术,X 线计算机
英文关键词:animal model  Pulmonary disease, chronic obstructive  tomography, X-ray computed
基金项目:
作者单位E-mail
张迪 海军军医大学附属长征医院,上海 200003 rinzy369@ 163.com 
夏艺 海军军医大学附属长征医院,上海 200003  
范丽 海军军医大学附属长征医院,上海 200003  
刘士远 海军军医大学附属长征医院,上海 200003  
管宇 海军军医大学附属长征医院,上海 200003 guan.yu8635@ 163.com 
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中文摘要:
      目的 通过烟熏法、蛋白酶滴注及两者相结合的方法构建大鼠慢性阻塞性肺疾病( chronic obstructive pulmonary disease, COPD)模型,并从炎症水平、影像及病理等方面评价造模效果,对三种建模方法进行比较。 方法 使用烟熏、蛋白酶滴注及两者相结合的方法进行 COPD 大鼠造模,每组大鼠分别为 60 只、30 只、30 只,同时设置对照组 20 只。 每周对大鼠进行体重测量。 烟熏组及对照组大鼠于造模 24 h,1、2、4、8、12、16、20、24 周,蛋白酶组及蛋白酶+烟熏组大鼠于造模 24 h,1、2、4、8、12 周接受细胞因子检测、Micro-CT 检查及病理检查。 采用方差分析或 Kruskal-Wallis H 检验进行统计分析。 结果 第 7 周起烟熏组大鼠及蛋白酶+烟熏组大鼠体重增长 较对照组明显减缓(P< 0. 05)。 蛋白酶组及蛋白酶+烟熏组大鼠第 24 h、1、2、4 周的白细胞介素-10 水平显著低于对照组(P< 0. 05)。 蛋白酶组及蛋白酶+烟熏组大鼠第 24 h 的基质金属蛋白酶- 9 浓度显著大于对照组( P< 0. 05)。 蛋白酶组、蛋白酶+烟熏组第 4 周及烟熏组第 8 周大鼠在 Micro-CT 图像及病理图像上均可观察到肺气肿改变。 结论 使用烟熏、蛋白酶及蛋白酶+烟熏的方法均可成功构建大鼠 COPD 模型。 Micro-CT 可灵敏真实的反应肺部病理改变。
英文摘要:
      Objective To establish rat models of chronic obstructive pulmonary disease (COPD) with smoking, protease instillation and smoking + protease instillation, and to evaluate and compare inflammation, imaging and pathology between these groups. Methods Rat models of COPD were established with smoking, protease instillation and smoking + protease instillation. There were 60 rats in the smoking group, 30 rats in the protease group and 30 rats in the smoking + protease group. The control group contained 20 rats. Body weight was measured every week. In the smoking and control groups, five and two rats were euthanized, respectively at 24 h, and weeks 1, 2, 4, 8, 12, 16, 20 and 24 during the modeling process, and in the protease and smoking + protease groups, five rats were euthanized in each group at 24 h, and weeks 1, 2, 4, 8 and 12. Rat lung tissues subjected to cytokine detection, micro-CT examination and pathological examination. Results The weight gain of rats in the smoking and smoking + protease group was significantly slower than the control group at week 7 (P< 0. 05). The levels of IL-10 at 24 h, and weeks 1, 2 and 4 were significantly lower in the protease group and the smoking + protease group compared with the control group (P< 0. 05). The concentration of MMP-9 at 24 h was significantly higher in the protease group and the smoking + protease group than the control group (P< 0. 05). Emphysema was observed on the micro-CT images and pathological images in the protease group and smoking + protease group at week 4, and in the smoking group at week 8. Conclusions Rat models of COPD can be successfully established by smoking, protease instillation and smoking + protease instillation. Micro-CT reflects changes in lungs with high sensitivity and validity.
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