首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
纪丽献,段新旺,牛海涛.羟氯喹对狼疮小鼠脾淋巴细胞的影响[J].中国实验动物学报,2020,28(2):0.
羟氯喹对狼疮小鼠脾淋巴细胞的影响
Effect of hydroxychloroquine on spleen lymphocytes in lupus mice
投稿时间:2019-10-08  
DOI:10. 3969 / j.issn.1005-4847. 2020. 02. 006
中文关键词:  系统性红斑狼疮  羟氯喹  淋巴细胞
英文关键词:systemic lupus erythematosus  hydroxychloroquine  lymphocytes
基金项目:
作者单位E-mail
纪丽献 南昌大学第二附属医院风湿免疫科,南昌 330006 18370959842@ 163.com 
段新旺 南昌大学第二附属医院风湿免疫科,南昌 330006 13970085678@ 163.com 
牛海涛 国家卫生健康委员会人类疾病比较医学重点实验室, 中国医学科学院医学实验动物研究所,国家中医药管理局,北京 100021 niuhaitao@ cnilas.org 
摘要点击次数: 196
全文下载次数: 23
中文摘要:
      目的 观察羟氯喹对系统性红斑狼疮小鼠(TC 小鼠)外周免疫器官(脾)淋巴细胞分布的影响。 方法 将 30 周龄的雌性 TC 小鼠随机分配,实验组经灌胃给予羟氯喹治疗处理[10 mg / ( kg·d)],对照组经灌胃给予 生理盐水处理[10 mg / (kg·d)],共处理 5 周。用酶联免疫吸附试验(ELISA)检测小鼠外周血抗 dsDNA 抗体滴度水平,苏木精-伊红(HE)染色法检测肾组织病理学改变,流式细胞术检测小鼠脾淋巴细胞比例。 结果 与对照组相比,实验组小鼠肾组织病理学评分显著低于对照组(P< 0. 05);实验组脾 B 淋巴细胞和生发中心 B 淋巴细胞比例显著低于对照组(P< 0. 05);实验组脾调节性 T 淋巴细胞(Treg)比例显著高于对照组(P< 0. 05);两组小鼠抗 dsDNA 抗体水平变化及 Th17 淋巴细胞比例变化无明显差异(P> 0. 05)。 结论 羟氯喹对狼疮小鼠外周免疫器官淋巴细胞的免疫应答具有调节作用并且可缓解 TC 小鼠肾组织病理损伤。
英文摘要:
      Objective To observe the effect of hydroxychloroquine on lymphocyte distribution in the peripheral immune organs ( spleen) of systemic lupus erythematosus mice (TC mice). Methods Thirty-week-old femaleTC mice were randomly assigned to two groups: the experimental group was treated with hydroxychloroquine [10 mg / (kg·d)] and the control group was treated with normal saline [10 mg / ( kg·d)] for 5 weeks. Enzyme-linked immunosorbent assay was used to detect the titer of anti-dsDNA antibody in the peripheral blood of mice, Hematoxylin-eosin staining was used to detect histopathological changes in kidneys, and flow cytometry was used to detect the proportion of lymphocytes in the spleen of mice. Results The histopathological score of kidneys in the experimental group was significantly lower than that in the control group ( P< 0. 05). The proportions of splenic B lymphocytes and germinal center B lymphocytes in the experimental group were significantly lower than that in the control group (P< 0. 05). The proportion of splenic regulatory Tlymphocyte (Treg) in the experimental group was significantly higher than that in the control group (P< 0. 05). There were no significant difference in the levels of anti-dsDNA antibody and the T helper cell 17 ( Th17) lymphocyte proportions between groups (P > 0. 05 ). Conclusions Hydroxychloroquine has a regulatory effect on the immune response of lymphocytes in peripheral immune organs of lupus mice and alleviated the pathological damage in kidney tissues of TCmice.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 1575184 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb2@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码