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邵芳冰,房军帆,王思思,孙海榉,项璇儿,方剑乔,杜俊英.慢性炎性痛模型大鼠诱发焦虑抑郁样情绪行为观察[J].中国实验动物学报,2020,28(2):0.
慢性炎性痛模型大鼠诱发焦虑抑郁样情绪行为观察
Observation of the anxiety and depression-like behavior induced by chronic inflammatory pain in rats
投稿时间:2019-08-27  
DOI:10. 3969 / j.issn.1005-4847. 2020. 02. 003
中文关键词:  慢性炎性痛  焦虑  抑郁  痛情绪  行为学  大鼠
英文关键词:chronic inflammatory pain  anxiety  depression  pain affect  behavior  rat
基金项目:
作者单位E-mail
邵芳冰 浙江中医药大学第三临床医学院 针灸神经生物学实验室,浙江杭州 310053 475073482@ qq.com 
房军帆 浙江中医药大学第三临床医学院 针灸神经生物学实验室,浙江杭州 310053  
王思思 浙江中医药大学第三临床医学院 针灸神经生物学实验室,浙江杭州 310053  
孙海榉 浙江中医药大学第三临床医学院 针灸神经生物学实验室,浙江杭州 310053  
项璇儿 浙江中医药大学第三临床医学院 针灸神经生物学实验室,浙江杭州 310053  
方剑乔 浙江中医药大学第三临床医学院 针灸神经生物学实验室,浙江杭州 310053 fangjianqiao7532@ 163.com 
杜俊英 浙江中医药大学第三临床医学院 针灸神经生物学实验室,浙江杭州 310053 dujunying0706@ 163.com 
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中文摘要:
      目的 动态观察弗氏完全佐剂(CFA)诱导慢性炎性痛模型大鼠痛情绪的改变情况,探索慢性炎性痛模型大鼠诱导焦虑、抑郁情绪的时间差异性。 方法 所有实验大鼠随机分为空白对照组和模型对照组,模型对照组于足底注射 CFA 建立慢性炎性痛模型。 动态观察各组大鼠造模前、造模后 1,3,7,18 和 26 d 机械缩足阈变化 (PWTs);观察造模后 28 d 旷场实验(OF),29 d 高架 O 迷宫实验(EZM),30 d 新奇食物抑制实验(NSF),造模后 12,20 和 31 d 大理石掩埋实验(MBT),造模后 17,25 和 35 d 糖水偏爱度实验(SPT)中大鼠行为改变情况。 结果 造模后 1 d,模型对照组大鼠 PWTs 显著降低(P < 0. 01),且在整个实验过程中均显著低于空白对照组(P < 0. 01)。 模型对照组在 OF 中较空白对照组大鼠的中央区运动距离、中央区停留时间、中央区进入次数显著减少( P < 0. 01);在 EZM 中较空白对照组大鼠的开放臂运动距离,开放臂停留时间,开放臂进入次数显著减少(P < 0. 05); 在 NSF 中较空白对照组大鼠摄食潜伏期明显增加(P < 0. 05),且食物消耗量无明显差异(P > 0. 05);而 MBT 仅模 后 31 d,模型对照组较空白对照组大鼠大理石埋藏数量显著增加(P < 0. 05);SPT 中模后各时间点模型对照组与空白对照组大鼠比较均无明显差异(P > 0. 05)。 结论 CFA 诱发的慢性炎性痛模型大鼠在 4 周后可出现多种与焦 虑样情绪相关的动物行为改变,但在 5 周内均并未观察到与抑郁样情绪相关的动物行为改变。
英文摘要:
      Objective To dynamically observe the temporal differences of anxiety and depression-like behavior in rats with chronic inflammatory pain induced by complete Freund’ s adjuvant (CFA). Methods All rats were randomly divided into control and model groups. The model group rats was injected with CFA into the hind paws to establish the chronic inflammatory pain model. We dynamically observed the changes of paw withdrawal thresholds before modeling and at 1, 3, 7, 18, and 26 days after modeling. In addition, behavioral changes were observed in an open field test at 28 days, in an elevated zero maze test at 29 days, in a novelty-suppressed feeding test at 29 days, and in a marble-burying test at 12, 20, and 31 days, as well as through a sucrose preference test after modeling at 17, 25, and 35 days. Results Compared with the control group, the paw withdrawal threshold of the model group significantly decreased after 1 day (P< 0. 01) and was significantly lower than the control group throughout the experiment (P< 0. 01). Compared with the control group, the distance in the central area, time in the central area, and number of central zone entries of the model group were significantly reduced in the open field (P< 0. 01). Compared with the control group, the distance in open arm, time in open arm, and number of the open arm entries of the model group were significantly reduced in the elevated zero maze (P< 0. 05). The latency of food of the model group was significantly increased ( P< 0. 05), and there was no significant difference in food consumption in the novelty-suppressed feeding (P> 0. 05). In the marble-burying test, the number of buried marbles in the model group increased significantly compared with the control group ( P < 0. 05) 31 days after modeling. There was no significant difference between the control group and the model group at any time point in the sucrose preference test (P> 0. 05). Conclusions Chronic inflammatory pain induced by CFA can lead to a variety of changes in the anxiety-like behavior of animals after 4 weeks, while no changes in the depression-like behaviors of animal was seen before 5 weeks.
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