Since the publication of special reviews and commentaries on zebrafish ( Danio rerio) in Science, Nature, and other journals in 1994, and completion of the whole genome sequencing of wild type (AB strain) zebrafish in 2009, the zebrafish has become a well-recognized new model organism. The remarkable advantages of zebrafish in the research and assessment of developmental toxicity and teratogenicity includes its transparency, high throughput, and rapid, high-efficiency, and low-cost screening. Zebrafish have been extensively used to screen the active components of traditional Chinese medicine for toxicological and pharmaceutical investigations, as well as for large-scale new drug screening of small molecule compounds. With the implementation of the " 3R principle (Reduction,Replacement,Refinement)" , the zebrafish has been proposed as an alternative animal model for developmental toxicity and teratogenicity tests. The recent progress of developmental toxicity and teratogenicity assessments in zebrafish is reviewed in this report.