一种产后小鼠束缚应激模型的造模方法以及束缚装置的制作
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复旦大学附属金山医院,上海 201500

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Method for constructing a postpartum mouse immobilization stress model and restraint device
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Jinshan Hospital, Fudan University, Shanghai 201500,China

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    目的 自制小鼠束缚装置,建立产后小鼠束缚应激模型供相关应激实验研究。 方法 选择规格适 合小鼠身型的离心管,制备简易小鼠束缚装置。在小鼠产后第 2 天,使用该装置固定产后母鼠于装置内,限制其行动。 在母子分离的基础上,每天给予母鼠 3 h 的束缚应激,连续造模 21 d 即可制备产后小鼠束缚应激模型。 结果 该产后小鼠束缚应激模型,其胃、下丘脑以及血清内 ghrelin 表达量增加,5-HT2 受体等基因表达量减少,并且具有运动量减少等抑郁样症状和子代生长发育迟缓现象。 结论 本文介绍产后小鼠束缚应激模型,经过多次实验证明,造模结果具有良好的稳定性和可重复性,可供相关小鼠束缚应激实验研究。

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    Objective A hand-made mouse restraint device was designed to establish an immobilization stress model for related stress experiments. Methods A centrifuge tube with a size suitable for the mouse body was used to prepare a mouse restraint device. The mouse restraint device was applied to maternal mice for 3 hours every day for 21 days starting 2 days after delivery to stimulate repeated immobilization stress. The maternal mice were separated from their offspring after delivery. Results In this postpartum mouse immobilization stress model, ghrelin expression was increased in the stomach, hypothalamus and serum, 5-HT2 receptor expression was decreased, and depression-like symptoms, such as decreased exercise and offspring growth retardation, were observed. Conclusions A new device is constructed to establish an immobilization stress model in postpartum mice. Results from repeated experiments show good stability and reproducibility, suggesting that this model can be used to study immobilization stress in mice.

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陈漫漫,高鹏飞.一种产后小鼠束缚应激模型的造模方法以及束缚装置的制作[J].中国实验动物学报,2020,28(1):100~107.

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  • 收稿日期:2019-08-21
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  • 在线发布日期: 2020-04-01
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