静脉注射针与自制PE-50 管针头用于大鼠阴茎海绵体内压测定的比较
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(1. 浙江中医药大学第二临床医学院,杭州 310053; 2. 南通大学附属医院泌尿男科,江苏南通 226001;3. 浙江中医药大学动物实验研究中心,杭州 310053; 4. 浙江中医药大学附属第二医院中心实验室,杭州 310005;5. 浙江中医药大学附属第二医院泌尿外科,杭州 310005; 6. 浙江中医药大学泌尿男科研究所,杭州 310053)

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Comparison of the use of intravenous infusion needle and self-made PE-50 tube needle in the measarement of intracavernosal pressure in rat penis
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(1. the Second Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou 310053, China.2. Department of Urology and Andrology, Affiliated Hospital of Nantong University, Nantong 226001.3. Laboratory Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053.4. Central Laboratory, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310005.5. Department of Urology, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310005.6. Institute of Urology and Andrology, Zhejiang Chinese Medical University, Hangzhou 310053)

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    摘要:

    目的 探讨电刺激阴茎海绵体神经检测海绵体内压方案,并主要比较两种置管材料(PE-50 管针头与静脉输注针)用于检测阴茎海绵体内压(intracavernous pressure,ICP)的差异?方法 选取SPF 级雄性Sprague-Dawley 大鼠36 只,随机选择16 只作为对照组,余20 只为实验组,予腹腔注射链脲佐菌素60 mg/ kg,饲养8 周后筛选出16 只I 型糖尿病大鼠?对照组与糖尿病组再分为使用PE-50 管针与静脉输注针组记录ICP 各8 只?Masson三色染色与阿扑吗啡实验证实糖尿病ED 大鼠模型造模成功?证实模型建立成功后,进行阴茎海绵体内压检测,比较PE-50 管针与静脉输注针记录的相关勃起功能数据?结果 Masson 三色染色显示糖尿病模型组较对照组阴茎海绵体平滑肌面积减少?胶原面积增多?阴茎海绵体平滑肌面积/ 胶原面积减少,差异具有统计学意义( P < 0. 05)?糖尿病模型组较对照组勃起次数明显减少( P < 0. 01)?正常组和糖尿病组使用PE-50 管针与静脉输注针记录的峰值ICP?ICP/ 平均动脉压(mean arterial pressure,MAP)?曲线下面积(area under curve,AUC) 无统计学意义( P >0. 05),而起峰速率(slope)有统计学意义( P < 0. 05 或 P < 0. 01)?结论 电刺激阴茎海绵体神经记录海绵体内压时,PE-50 管针头与静脉输注针头均适用于阴茎海绵体内压测定?

    Abstract:

    Objective To explore the method for detecting intracavernosal pressure ( ICP) under electrostimulation of the cavernous nerve, and to compare the difference between two catheter materials (PE-50 tube needle and intravenous infusion needle) for measurent of ICP. Methods Thirty-six male Sprague-Dawley rats were randomly selected: 16 were randomly selected as the control group, and the remaining 20 were used as the experimental group. Intraperitoneal injections of streptozotocin (60 mg/ kg) were given. After 8 weeks, 16 mice of type 1 diabetes were screened. The control and diabetic groups were further divided into two subgroups, with eight rats in each subgroup. ICP was evaluated using PE- 50 tube needles and other eight rats with intravenous infusion needles. Masson trichrome staining and apomorphine experiment confirmed that the diabetic erectile dysfunction (ED) rat model was successfully developed. After confirming the successful establishment of the model, the ICP tests of the penis were performed, and the erectile function data recorded using the PE-50 tube needle and the intravenous infusion needle were compared. Results Masson trichrome staining showed that the diabetic model group had a smaller area of corpus cavernosum smooth muscle, increased collagen area, and decreased ratio of corpus cavernosum smooth muscle area/ collagen area, all of which were statistically significant ( P < 0. 05). The number of erections in the diabetic model group was significantly lower than that in the control group ( P < 0. 01). Maximum ICP, ICP/ mean arterial pressure, and the area under the curve recorded in the normal and diabetic groups using PE-50 tube needles and intravenous infusion needles were not statistically significantly different ( P > 0. 05), but the slopes were significantly different ( P < 0. 05 or P < 0. 01). Conclusion Both the PE-50 tube needle and intravenous infusion needle can be used in the measurement of ICP during electrostimulation of the cavernous nerve.

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叶妙勇,赵凡,马轲,张利棕,方明笋,寿旗扬,马寅锋,黄文杰,吕伯东.静脉注射针与自制PE-50 管针头用于大鼠阴茎海绵体内压测定的比较[J].中国实验动物学报,2019,27(6):753~759.

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  • 收稿日期:2019-05-05
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  • 在线发布日期: 2020-01-07
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