首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
郭军雄,汪斌,马丽,康万荣,许小敏,徐生刚.p38 MAPK 在腹泻型肠易激综合征大鼠中的变化及其免疫调控作用[J].中国实验动物学报,2019,27(6):709~715.
p38 MAPK 在腹泻型肠易激综合征大鼠中的变化及其免疫调控作用
Immunoregulatory effects of the p38 MAPK-signaling pathway in rats with diarrhea-predominant irritable bowel syndrome
投稿时间:2019-04-12  
DOI:10. 3969 / j.issn.1005-4847. 2019. 06. 004
中文关键词:  肠易激综合征  腹泻型  p38 MAPK 信号通路  白细胞介素1β  白细胞介素6  肿瘤坏死因子α  大鼠
英文关键词:irritable bowel syndrome  diarrhea-predominant  p38 MAPK signaling pathway  IL-1β  IL-6  TNF-α  rats
基金项目:
作者单位E-mail
郭军雄 河西学院医学院,河西学院丝绸之路中医药研究中心,河西学院中西医结合研究所,甘肃张掖 734000 gjxiong1124@ 126.com 
汪斌 河西学院医学院,河西学院丝绸之路中医药研究中心,河西学院中西医结合研究所,甘肃张掖 734000  
马丽 河西学院医学院,河西学院丝绸之路中医药研究中心,河西学院中西医结合研究所,甘肃张掖 734000  
康万荣 甘肃中医药大学,兰州 730000  
许小敏 河西学院医学院,河西学院丝绸之路中医药研究中心,河西学院中西医结合研究所,甘肃张掖 734000  
徐生刚 河西学院医学院,河西学院丝绸之路中医药研究中心,河西学院中西医结合研究所,甘肃张掖 734000  
摘要点击次数: 96
全文下载次数: 18
中文摘要:
      目的 探讨p38 MAPK 信号在腹泻型肠易激综合征大鼠中的免疫调控作用?方法 将40 只SPF级Wistar 大鼠(雌雄各半),随机分为D-IBS 模型Ⅰ(造模7 d)组?Ⅱ(造模14 d)?III(造模21 d)组及空白对照组,每组10 只?模型组采用慢性束缚联合番泻叶灌胃法建模,空白对照组灌服同等体积的纯净水?D-IBS 大鼠分别于建模第7 天?第14 天和第21 天剖杀,空白对照组于第21 天剖杀?心脏采血,用酶联免疫吸附测定法(ELISA)检测各组大鼠血清IL-1β?IL-6 和TNF-α 含量;剖取结肠组织,HE 染色观察大鼠结肠组织形态学,免疫组化法测大鼠结肠组织中p38 MAPK 的蛋白表达?结果 与空白对照组比较,模型组大鼠血清IL-1β?IL-6 和TNF-α 含量和结肠p38 MAPK 蛋白表达阳性率增高( P <0. 05, P <0. 01),且p38 MAPK 的表达与IL-1β?IL-6 和TNF-α 的水平呈正相关?结论 慢性束缚联合番泻叶灌胃法复建的D-IBS 大鼠模型,可能存在通过激活p38 MAPK 信号通路,可上调p38 MAPK 的表达,促进IL-1β?IL-6 和TNF-α 的释放,诱发肠黏膜低度炎症反应?
英文摘要:
      Objective To investigate the immunoregulatory effect of P38 mitogen activated protease ( p38 MAPK) signals in rats with diarrhea-predominant irritable bowel syndrome (D-IBS). Methods Forty SPF Wistar rats (half male and half female) were randomly divided into a control group, and D-IBS model I (7 d), II (14 d) and III (21 d) groups, with 10 rats in each group. Rats in the model groups were developed through chronic restraint and senna decoction gavage, whereas the control rats were gavaged with the same volume of pure water. The D-IBS rates were killed on the seventh, fourteenth and twenty-first days, respectively. Rats in the control group were killed on the twenty-first day. The serum levels of IL-1β, IL-6 and TNF-α were measured by ELISA. The pathological changes in colon tissue were observed with hematoxylin and eosin (H&E) staining, and the p38 MAPK protein expression in the colon tissue was assessed with immunohistochemistry. Results Compared with the control group, the IL-1β, IL-6 and TNF-α levels in serum, and the p38 MAPK protein expression in colon tissue of the model groups were significantly higher ( P <0. 05, P <0. 01). The expression of p38 MAPK was positively correlated with IL-1β, IL-6 and TNF-α. Conclusions The D-IBS rat model, developed through chronic restraint and senna decoction gavage, may have upregulation of the expression of p38 MAPK, promoting the release of IL-1β, IL-6 and TNF-α, and inducing mild inflammation of the intestinal mucosa by activating the p38 MAPK signal pathway.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 1390205 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb2@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码