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马晓中,张韶鹏,王菁,姜楠,陈庆良.β-氨基丙腈诱导鼠夹层动脉瘤动物模型的建立[J].中国实验动物学报,2019,27(5):644~650.
β-氨基丙腈诱导鼠夹层动脉瘤动物模型的建立
β-Aminopropionitrile-induced rodent models of aortic dissecting aneurysm
投稿时间:2019-03-26  
DOI:10. 3969 / j.issn.1005-4847. 2019. 05. 015
中文关键词:  动脉瘤  β-氨基丙腈  小鼠
英文关键词:aortic dissecting aneurysm  β-aminopropionitrile  mouse
基金项目:
作者单位E-mail
马晓中 天津医科大学研究生院,天津 300070 zsp0810@ yeah.net 
张韶鹏 天津市胸科医院心外科,天津市心血管病研究所,天津,300222  
王菁 天津市胸科医院病理科,天津 300222  
姜楠 天津市胸科医院心外科,天津市心血管病研究所,天津,300222  
陈庆良 天津市胸科医院心外科,天津市心血管病研究所,天津,300222 qingliang1971@ 126.com 
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中文摘要:
      目的 探索β-氨基丙腈(β-Aminopropionitrile,BAPN)对鼠大动脉壁的影响,并对比人类夹层动脉瘤发病特点,以期进一步探索符合人类疾病特征的夹层动脉瘤动物模型?方法 在实验动物饮用水中混入BAPN,配成浓度为0. 2%?0. 4%?0. 6%的BAPN 溶液,4 ~ 5 周龄SPF 级SD 大鼠和3 周龄SPF 级C57BL/6 小鼠饲养7 周?实验结束或动物死亡后将其解剖,分离其大动脉,观察大体变化?将大动脉分为升主动脉?降主动脉?腹主动脉肾动脉上段和腹主动脉肾动脉下段4 部分,截取每段血管横断面进行HE 染色,并测量其血管内径?中膜面积等各项指标?同时,留取行开胸手术的A 型夹层动脉瘤患者大动脉进行HE 染色,观察其病理改变,与发生夹层动脉瘤的鼠大动脉进行比较?结果 1)BAPN 可显著影响大鼠或小鼠采水量及体重增长?2)BAPN 可致大鼠或小鼠大动脉扩张,中膜增厚,弹性蛋白减少?排列紊乱,其病理改变符合人类夹层动脉瘤病理改变特征?3)浓度为0. 4%的BAPN 溶液模型成功率最高?结论 C57BL/6 小鼠夹层动脉瘤动物模型可作为一种简便?经济?有效的动物模型进行下一步研究; SD 大鼠发生肠破裂?脊柱侧弯等系统病理改变的比例高于夹层动脉瘤发生率,其作为夹层动脉瘤动物模型尚需进一步探索?
英文摘要:
      Objective The aim of this study was to investigate the effect of lysyloxidase inhibitor betaaminopropionitrile(BAPN) on the aortic wall in rats, to analyze the gross and pathological changes of arterial and othertissues of rats treated with BAPN at different concentrations, and to compare with the characteristics of human dissected aneurysm. Methods Eighteen SPF SD rats (4-5-week old) were divided into three groups: SD-0. 2 (Group A), SD-0. 4(Group B), and SD-0. 6 (Group C). The groups A, B and C were given 0. 2%, 0. 4%, and 0. 6% BAPN solution,respectively, as drinking water for seven weeks. Forty SPF C57BL/6 mice (3-week old) were randomly divided into four groups: C57-0. 2 (Group D), C57-0. 4 (Group E), C57-0. 6 (Group F) and the control group and given 0. 2%, 0. 4%,or 0. 6% BAPN or distilled water as drinking water, respectively, for seven weeks. All experimental animals were free to drink water. The daily water intake was recorded and the weight was measured once a week. Rats that died during theexperiment or survived after the experiment were dissected. The aortas were dissected and visually examined. The aorta was divided into four parts: ascending aorta, descending aorta, abdominal aorta above the renal artery and abdominal aorta under the renal artery. The aortic tissues were cut into 4 μm sections and stained with hematoxylin and eosin for pathological examination. The vascular diameter, and area of the tunica media were measured by image analysis. The pathological changes of aorta and dissecting aneurysm of 10 patients were also observed and compared with the rat aorta with dissecting aneurysms. Results BAPN significantly affected the water intake and weight gain of rats or mice. BAPN caused thickening of the tunica media in the aorta of rats or mice, and reduction and disordered arrangement of elastic protein. Their pathological changes were similar to the pathological changes of human aneurysms. Conclusions The incidence of dissecting aneurysm in C57BL/6 mice was much higher than that of SD rats, indicating that mice may be an ideal animal model for further study. In SD rats, the rate of pathological changes in other systems, such as intestinal rupture and scoliosis, was higher than that in the dissection aneurysm. Further exploration for SD rats as an animal model of aortic dissecting aneurysm is needed.
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