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韩志刚,何明忠,何丽雯,曹科,谭毅,谭冬梅.富硒米糠代替有机硒对实验仔兔生长性能和抗氧化功能的影响[J].中国实验动物学报,2019,27(5):637~643.
富硒米糠代替有机硒对实验仔兔生长性能和抗氧化功能的影响
Using selenium-enriched rice bran to replace organic selenium and its effect on growth performance and antioxidant function in weaned rabbits
投稿时间:2019-04-01  
DOI:10. 3969 / j.issn.1005-4847. 2019. 05. 014
中文关键词:    富硒米糠  仔兔  生长性能  抗氧化
英文关键词:selenium  selenium-enriched rice bran  weaned rabbit  growth performance  antioxidation
基金项目:
作者单位E-mail
韩志刚 重庆医科大学实验动物中心, 重庆 400016 5753891@ qq.com 
何明忠 重庆医科大学实验动物中心, 重庆 400016  
何丽雯 重庆医科大学实验动物中心, 重庆 400016  
曹科 重庆医科大学实验动物中心, 重庆 400016  
谭毅 重庆医科大学实验动物中心, 重庆 400016  
谭冬梅 重庆医科大学实验动物中心, 重庆 400016 dongmei_tan@ 126.com 
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中文摘要:
      目的 研究富硒米糠代替有机硒添加对仔兔生长性能和抗氧化功能的影响?方法 将60 只35 日龄断奶仔兔随机分为普通米糠组?有机硒组?富硒米糠组,每组20 只,雌雄各半,分别饲喂普通米糠饲料?普通米糠加有机硒饲料?富硒米糠饲料,共饲喂30 d,记录每日饲料消耗量?分别于实验第0?5?10?15?20?25?30 天称量实验兔体重,计算每组实验兔料重比?饲喂结束后,采集各组实验兔血清,检测血糖?尿酸?总蛋白?白蛋白?球蛋白含量?白蛋白/ 球蛋白比及血清谷胱甘肽过氧化物酶(GSH-Px)活性?超氧化物歧化酶活性(SOD)?过氧化氢酶(CAT)活性和丙二醛(MDA)含量;采集各组实验兔肝?脾?肾并称重,计算各组组内脏器指数,并检测肾硒含量?结果 有机硒组和富硒米糠组的日增重?料重比?脏器指数?肾硒含量以及上述血液生化指标同普通米糠组相比,差异均无显著性( P > 0. 05),但富硒米糠组的GSH-Px 和SOD 活性?有机硒组的GSH-Px 和CAT 活性较普通米糠组均有显著增加( P < 0. 05),两组的MDA 含量较普通米糠组均有明显降低( P < 0. 05),且富硒米糠组和有机硒组的抗氧化能力相当?结论 富硒米糠对仔兔生长性能和抗氧化功能的促进作用与有机硒相当,可代替有机硒作为仔兔饲料硒的来源?
英文摘要:
      Objective To investigate the effects of selenium-enriched rice bran on the growth performance andantioxidant function in weaned rabbits. Methods Sixty 35-day-old weaned rabbits were randomly divided into three groups(n =20, 1∶1 male ∶female) and were fed with feed added ordinary rice bran, ordinary rice bran plus selenium-enrichedyeast or selenium-enriched rice bran for 30 days. Feed consumption was recorded daily and the rabbits were weighed at 0 d,5 d, 10 d, 15 d, 20 d, 25 d and 30 d, respectively. After 30 days, serum was collected and the content of blood glucose,uric acid, total protein, albumin, globulin, and albumin/ globulin ratio were measured by the IDEXX Catalyst Dx Analyzer.The activity of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and the content ofmalondialdehyde (MDA) were detected by assay kits. The liver, spleen and kidney were weighed, organ index wascalculated, and the selenium content in the kidney was determined. Results There were no significant differences in dailyweight gain, feed conversion ratio, organ index, kidney selenium content and the blood biochemical indexes among thethree groups ( P >0. 05). However, the activity of GSH-Px and SOD in the selenium-enriched rice bran group and theactivity of GSH-Px and CAT in the organic selenium group were significantly higher than that in the ordinary rice bran group( P <0. 05). The MDA content in these two groups was significantly lower than that of the ordinary rice bran group ( P <0. 05). In addition, the antioxidant capacity was comparable between the selenium-enriched rice bran group and organicselenium group. Conclusions Selenium in selenium-enriched rice bran plays a positive role in promoting growth andresisting oxidation in weaned rabbits, which is similar to the selenium in yeast. These findings indicate selenium-enriched rice bran can be used as a selenium source of feed for weaned rabbits.
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