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贾欢欢,罗晓玲,高洪彬,梁十,罗挺,陈结文,何积存,黄庆宇,李永锋,卢丽,陈梅丽.自发性糖尿病食蟹猴的诊断及其骨微结构的改变[J].中国实验动物学报,2019,27(5):626~630.
自发性糖尿病食蟹猴的诊断及其骨微结构的改变
Diagnosis of and bone microstructure in spontaneous diabetic cynomolgus monkeys
投稿时间:2019-04-11  
DOI:10. 3969 / j.issn.1005-4847. 2019. 05. 012
中文关键词:  食蟹猴  糖尿病合并骨质疏松  动物模型  糖耐量  骨结构
英文关键词:cynomolgus monkeys  diabetic osteoporosis  animal model  glucose tolerance  bone structure
基金项目:
作者单位E-mail
贾欢欢 广东省实验动物监测所 jhh@ gdlami.com 
罗晓玲 1. 广东药科大学生命科学与生物制药学院,广州 510006
2. 广州春盛生物研究院有限公司,广州 510940 
 
高洪彬 广东省实验动物监测所  
梁十 广东省实验动物监测所  
罗挺 广东省实验动物监测所  
陈结文 广州春盛生物研究院有限公司,广州 510940  
何积存 广州春盛生物研究院有限公司,广州 510940  
黄庆宇 广州春盛生物研究院有限公司,广州 510940  
李永锋 广州春盛生物研究院有限公司,广州 510940  
卢丽 1. 广东药科大学生命科学与生物制药学院,广州 510006
2. 广州春盛生物研究院有限公司,广州 510940 
541608180@ qq.com 
陈梅丽 广东省实验动物监测所 1071138433@ qq.com 
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中文摘要:
      目的 对自发性糖尿病食蟹猴进行诊断,并分析其骨微结构的变化,以期建立自发性糖尿病合并骨质疏松动物模型?方法 选取13~20 岁的自发性糖尿病食蟹猴4 只,以年龄相近的动物作为对照;利用快速血糖仪检测空腹血糖及糖耐量,生化仪检测血清中相关生化指标,以此对自发性糖尿病食蟹猴进行诊断;利用Micro-CT测量股骨下段和胫骨上段骨结构,分析自发性糖尿病食蟹猴骨微结构的变化?结果 自发性糖尿病组食蟹猴的空腹血糖大于8. 0 mmol/ L,糖耐量试验120 min 时血糖大于10. 0 mmol/ L,且TG?LDL-C 与对照组比较显著升高,HDL-C 及Crea 显著低于对照组,TC 及BUN 无明显差异,可确诊为自发糖尿病动物;Micro-CT 结果显示自发性糖尿病食蟹猴骨微结构破坏明显,呈骨质疏松表现,且股骨下段和胫骨上段松质骨骨小梁面积?骨小梁体积及骨矿含量均显著低于对照组,而股骨下段和胫骨上段皮质骨两组间比较无明显差异?结论 自发性糖尿病食蟹猴与人类糖尿病患者有着相似的临床特征,可作为一种理想的糖尿病合并骨质疏松动物模型?
英文摘要:
      Objective To diagnose the spontaneous diabetic cynomolgus monkey and examine their bonemicrostructure, and establish an animal model of spontaneous diabetic osteoporosis. Methods Four spontaneous diabeticcynomolgus monkeys (13 to 20 years old) were included. Animals of similar ages were used as a control group. Fastingblood glucose and glucose tolerance were measured using a fast blood glucose meter. TC, LDL-C, HDL-C, TG, Crea, andBUN were measured to make the diagnosis of spontaneous diabetic cynomolgus monkeys. Micro-CT was used to measure thebone structure of distal femur and upper tibia. Results In the spontaneous diabetic group, the fasting blood glucose washigher than 8. 0 mmol/ L, and the blood glucose was higher than 10. 0 mmol/ L at 120 min of the glucose tolerance test. TGand LDL-C of the spontaneous diabetic cynomolgus monkeys were significantly higher than the control group, while HDL-Cand Crea were significantly lower than the control group ( P < 0. 05). There was no significant difference between TC andBUN, which could be diagnosed as spontaneously spontaneous diabetic cynomolgus monkeys. Micro-CT results showed thatthe distal femur and upper tibia of the spontaneous diabetic cynomolgus monkeys were damaged indicating osteoporosis.Moreover, the trabecular area, trabecular volume and bone mineral content of cancellous bone in the distal femur and uppertibia of the spontaneous diabetic cynomolgus monkeys were significantly lower than the control group. There were nosignificant differences in the cortical bone of the distal femur and upper tibia between the two groups. Conclusions The spontaneous diabetic cynomolgus monkeys have similar clinical characteristics with human patients, and may be used as an excellent animal model in studies of diabetic osteoporosis.
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