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赵国栋,陈晖,邵建翔,马晨,阿依先木·米吉提,艾则孜·阿布都克依木,李晓斌. L -精氨酸在实验室果蝇扩繁中的应用[J].中国实验动物学报,2019,27(5):612~618.
L -精氨酸在实验室果蝇扩繁中的应用
Application of L -arginine in laboratory Drosophila melanogaster propagation
投稿时间:2019-04-03  
DOI:10. 3969 / j.issn.1005-4847. 2019. 05. 010
中文关键词:  精氨酸  实验室  果蝇  化蛹数  羽化数
英文关键词: L -arginine  laboratory  Drosophila melanogaster  pupation number  feathering number
基金项目:
作者单位E-mail
赵国栋 新疆农业大学动物遗传育种与繁殖实验室,新疆乌鲁木齐 830052 616076990@ qq.com 
陈晖 新疆农业大学动物遗传育种与繁殖实验室,新疆乌鲁木齐 830052  
邵建翔 新疆农业大学动物遗传育种与繁殖实验室,新疆乌鲁木齐 830052  
马晨 新疆农业大学动物遗传育种与繁殖实验室,新疆乌鲁木齐 830052  
阿依先木·米吉提 新疆农业大学动物遗传育种与繁殖实验室,新疆乌鲁木齐 830052  
艾则孜·阿布都克依木 新疆农业大学动物遗传育种与繁殖实验室,新疆乌鲁木齐 830052  
李晓斌 新疆农业大学动物遗传育种与繁殖实验室,新疆乌鲁木齐 830052 172387243@ qq.com 
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中文摘要:
      目的 实验旨在研究在玉米琼脂培养基中添加 L -精氨酸,探究 L -精氨酸对果蝇化蛹时间?羽化时间?羽化率及雌雄比例的影响?方法 实验分为2 组,分别为对照组和实验组,对照组为基础培养基,实验组在基础培养基加1%的 L -精氨酸,每管中雌?雄1∶1放入7 对果蝇,每组12 个重复,接种72 h 后移除亲本,统计各组果蝇化蛹时间?羽化时间?羽化数及各组雌雄个数?结果 培养基中添加1%的 L -精氨酸,白眼(w1118 )?残翅(CyO)和野生型黑腹果蝇的化蛹数?羽化数分别比对照组提高313. 64%?303. 53%( P < 0. 01),157. 09%?182. 76%( P < 0. 01)和233. 33%?239. 03%( P < 0. 01),添加1%的 L -精氨酸后白眼和野生型果蝇的化蛹时间比对照组提前2 d?结论 在本实验条件下,1%的 L -精氨酸可提高实验室果蝇繁殖力,可应用于实验室果蝇的扩繁?
英文摘要:
      Objective To explore the effect of L -arginine on pupation time, eclosion time, eclosion rate andmale/ female ratio in Drosophila melanogaster. Methods Two groups of fruit flies were included in this study, the controlgroup received corn meal agar medium (basic medium) and the experimental group received 1% L -arginine added in thebasal medium. Seven pairs of fruit flies were placed in each tube, female: male=1∶1, 12 replicates in each group, and theparent flies were removed after 72 h. The time of pupation, the time of feathering, the number of feathering, and thenumber of males and females in each group were counted. Results After the addition of 1% L -arginine in the basicmedium, the numbers of pupation and feathering, and the feathering rate of the white eye (w1118 ), residual wing (CyO)and wild type Drosophila melanogaster were increased by 313. 64% and 303. 53% ( P < 0. 01), 157. 09% and 182. 76% ( P < 0. 01), and 233. 33% and 239. 03% ( P < 0. 01), respectively. In the group with addition of 1% L -arginine, thepupation time of the both white eye and wild type flies was 2 days earlier than the control group. Conclusions Addition of1% L -arginine in the basic medium, used under the conditions of this experiment, can increase the fertility of laboratory fruit flies and can be applied to the expansion of laboratory fruit flies.
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