首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
吕琛,张全伟,王琪,张勇,马友记,赵兴绪.金黄色葡萄球菌感染小鼠乳房炎模型的建立及IL-2 和IL-4 的变化[J].中国实验动物学报,2019,27(5):604~611.
金黄色葡萄球菌感染小鼠乳房炎模型的建立及IL-2 和IL-4 的变化
Establishment of a mouse model of mastitis with Staphylococcus aureus infection and its changes of IL-2 and IL-4 expression
投稿时间:2019-03-25  
DOI:10. 3969 / j.issn.1005-4847. 2019. 05. 009
中文关键词:  乳房炎  白介素因子2  白介素因子4  金黄色葡萄球菌  小鼠模型
英文关键词:mastitis  IL-2  IL-4  Staphylococcus aureus  mouse model
基金项目:
作者单位E-mail
吕琛 甘肃农业大学动物医学院,兰州 730070 695941433@ qq.com 
张全伟 甘肃农业大学生命科学技术学院,兰州 730070  
王琪 甘肃农业大学动物医学院,兰州 730070  
张勇 1. 甘肃农业大学动物医学院,兰州 730070
2. 甘肃农业大学生命科学技术学院,兰州 730070 
 
马友记 甘肃农业大学动物科技学院兰州 730070  
赵兴绪 1. 甘肃农业大学动物医学院,兰州 730070
2. 甘肃农业大学生命科学技术学院,兰州 730070 
zhaoxx@ gsau.edu.cn 
摘要点击次数: 40
全文下载次数: 11
中文摘要:
      目的 探讨炎症因子白细胞介素2(IL-2)和白细胞介素4(IL-4)在小鼠乳房炎发病过程中的变化情况?方法 选取12 只泌乳期雌鼠(BALB/ C),随机分为对照组(A 组),高浓度组(B 组),中浓度组(C 组)和低浓度组(D 组)?其中A 组注射生理盐水;B?C?D 组分别注射1. 2×105?1. 2×104?6. 0×103 CFU/ mL 不同浓度的金黄色葡萄球菌?采用H&E 染色法观察其病理变化,通过qRT-PCR?免疫组化法和Western blot 法检测小鼠乳腺组织中IL-2 和IL-4 mRNA 和蛋白水平的表达?结果 H&E 染色结果表明,随着金黄色葡萄球菌的浓度增加乳腺组织的病理学改变逐渐加重?通过免疫组化法?qRT-PCR 和Western blot 法发现,与A 组相比,B?C?D 组IL-2 的表达水平均明显增高( P < 0. 05),但随着感染菌浓度的增高其表达水平逐渐降低?同时,与A 组相比,B?C?D 组IL-4 的表达水平均明显增高( P < 0. 05),但随着感染菌浓度的增高其表达水平逐渐增高?结论 炎症因子IL-2?IL-4 参与了金黄色葡萄球菌致小鼠乳房炎的发生,对金黄色葡萄球菌性乳房炎的病理机制奠定了基础,为研究乳腺免疫机制提供科学的理论基础?
英文摘要:
      Objective To investigate the changes of inflammatory factors interleukin-2 (IL-2) and interleukin 4(IL-4) in the pathogenesis of mouse mastitis. Methods Twelve lactating female BALB/ C mice were randomly divided intothe control group (group A), high concentration group (group B), medium concentration group (group C) and lowconcentration group (group D). Group A was injected with normal saline. Groups B, C and D were injected with differentconcentrations of Staphylococcus aureus, 1. 2×105 CFU/ mL, 1. 2×104 CFU/ mL and 6. 0×103 CFU/ mL, respectively. Thepathological changes were observed using H&E staining. The expression of IL-2 and IL-4 mRNA and protein in the mousemammary glands were detected by qRT-PCR, immunohistochemistry and western blot. Results The pathologicalexamination showed that the pathological changes of breast tissue were gradually aggravated with the increase of theconcentration of S. aureus. Immunohistochemistry, qRT-PCR and western blot assays showed that the expression levels ofIL-2 in the groups B, C and D were significantly higher than that in the group A ( P < 0. 05) However, as the concentrationof infectious bacteria increased, the expression level of IL-2 gradually decreased. Compared with the group A, the groupsB, C and D showed significantly increased expression levels of IL-4 ( P < 0. 05), but the expression level increasedgradually with the increase of concentration of infectious bacteria. Conclusions The inflammatory factors IL-2 and IL-4 areinvolved in the development of mastitis caused by S. aureus, which lays a foundation for the pathologic mechanism of S.aureus mastitis and provide a theoretical scientific basis for studying the immune mechanism of breast.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 1302607 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb1@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码