中国仓鼠自发性2 型糖尿病基础代谢特征及相关基因的表达差异
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(1. 山西医科大学实验动物中心,太原 030000; 2. 山西省实验动物与人类疾病动物模型重点实验室,太原 030000)

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Q95-33

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Characteristics of basal metabolism and expression of related genes in spontaneous type 2 diabetic Chinese hamster
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(1. Laboratory Animal Center of Shanxi Medical University, Taiyuan 030000, China;2. Shanxi Key Laboratory of Experimental Animal Science and Animal Model of Human Diseases, Taiyuan 030000)

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    摘要:

    目的 探究自发性2 型糖尿病中国仓鼠糖脂代谢、体成分、昼夜运动及新陈代谢等基础代谢特征和相关基因在骨骼肌、肝中的表达情况。方法 根据中国仓鼠空腹血糖(FBG)和餐后血糖(PBG)值,选取对照组(FBG ≤ 4. 5 mmol/ L 且PBG < 6. 0 mmol/ L) 与糖尿病组(FBG ≥ 6. 0 mmol/ L 且PBG > 7. 0 mmol/L),测定动物体重、血糖、血脂、血清胰岛素含量及糖耐量,分析动物体成分,昼夜运动及新陈代谢特征,检测相关基因葡萄糖转运蛋白4 ( glucose transporter 4, Glut4) 和过氧化物酶体增殖激活受体-γ ( peroxisomeproliferative activated receptor-γ, Pparg)在骨骼肌和肝中的表达情况。结果 与对照组相比,中国仓鼠糖尿病组血糖、血脂含量增加,血清胰岛素含量和胰岛素抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR)增大,体脂率降低,摄食量和白天活动量增加,热量消耗增大。PPARG 在肝和骨骼肌中的mRNA和蛋白表达水平显著增加;GLUT4 在骨骼肌中的mRNA 和蛋白表达水平显著降低。结论 自发性2 型糖尿病中国仓鼠属于糖脂代谢异常,能产生胰岛素抵抗的非肥胖型2 型糖尿病动物模型,GLUT4 的下调可能与骨骼肌中异常的糖代谢及胰岛素抵抗有关,而上调的PPARG 可能有利于机体胰岛素抵抗状态的缓解。

    Abstract:

    Objective To explore the basic metabolic characteristics and expression of related genes in theskeletal muscle and liver of Chinese hamsters with spontaneous type 2 diabetes, including glucose and lipid metabolism,body composition, diurnal movement, metabolism, and the expression of relevant genes. Methods We regarded theChinese hamster with fasting blood glucose (FBG) higher than 6. 0 mmol/ L and post-prandial blood glucose (PBG) higherthan 7. 0 mmol/ L as diabetic based on the mean and 95% frequency distribution values of FBG and PBG. 12 hamsters atthe age of 48 weeks in diabetic and control groups, each group was half male and female. Animal weight, blood glucose,blood lipid, serum insulin content, and glucose tolerance were measured. Animal body composition, diurnal movement,and metabolic characteristics were analyzed by InAlyzer Dual Energy X-ray Animal Body Composition Analysis System,High-throughput Behavior Analysis System, and Open Circuit Calorimetric Metabolic System, respectively. The expressionof diabetes-related genes Glut4 and Pparg in skeletal muscle and liver were measured by Real-time PCR and Westernblotting. Results In the diabetic group, blood glucose and lipid, serum insulin, food intake, daytime activity, and heatconsumption were significantly increased, while the percentage of body fat was significantly decreased.Compared with thecontrol group, the mRNA and protein expressions of PPARG in the liver and skeletal muscle were significantly increased,and the mRNA and protein expressions of GLUT4 in skeletal muscle were significantly decreased in diabetic hamsters.Conclusions This type 2 diabetic Chinese hamster model exhibits non-obese, abnormal glucose and lipid metabolism, andinsulin resistance. The down-regulation of GLUT4 may be related to abnormal glucose metabolism and insulin resistance in skeletal muscle, while the up-regulated PPARG may alleviate insulin resistance in the liver.

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王璐,张锐虎,王晨阳,余婧婧,宋国华,王春芳,陈朝阳.中国仓鼠自发性2 型糖尿病基础代谢特征及相关基因的表达差异[J].中国实验动物学报,2019,27(4):508~515.

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  • 收稿日期:2019-01-21
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  • 在线发布日期: 2019-09-04
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