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李彦霖,谭思然,何丽雯,曹科,张倩,谭冬梅,谭毅.莫扎特K448 奏鸣曲高频段声波对小鼠抑郁模型干预治疗的分析[J].中国实验动物学报,2019,27(4):501~507.
莫扎特K448 奏鸣曲高频段声波对小鼠抑郁模型干预治疗的分析
Treatment with the high frequency of Mozart K448 in a mouse model of depression
投稿时间:2018-08-31  
DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 012
中文关键词:  音乐  频率  抑郁模型  小鼠
英文关键词:music  frequency  depression  mouse model
基金项目:
作者单位E-mail
李彦霖 重庆医科大学实验动物中心,重庆 400016 yanlinleeck@ 163.com 
谭思然 重庆医科大学实验动物中心,重庆 400016 962078353@ qq.com 
何丽雯 重庆医科大学实验动物中心,重庆 400016  
曹科 重庆医科大学实验动物中心,重庆 400016  
张倩 重庆医科大学实验动物中心,重庆 400016  
谭冬梅 重庆医科大学实验动物中心,重庆 400016  
谭毅 重庆医科大学实验动物中心,重庆 400016 tanyee66@ 126.com 
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中文摘要:
      目的 建立C57BL/6 小鼠抑郁模型,初步探究莫扎特K448 奏鸣曲中的高频段声波改善C57BL/6小鼠抑郁症状的效果。方法 1)慢性应激模型的建立:小鼠依据自主活动实验结果剔除活动次数差异较大者,其余分为空白组(n = 10)、模型组(n = 36),模型组经历5 周慢性温和不可预知刺激(chronic unpredictable and mildstress, CUMS),建立小鼠抑郁模型。(2)治疗干预:造模成功后,将模型组小鼠随机均衡分为模型对照组(n = 12)、氟西汀组(n =12)和音乐组(n =12)。氟西汀组每天腹腔注射盐酸氟西汀溶液(10 mg/ kg),其余两组注射等量的生理盐水。音乐组每天进行2 h 高频音乐干预,其余两组不进行音乐干预。干预持续2 周。(3)效果评价:实验前3 d及实验中每周称量体重并记录,实验第1 周、第5 周、第7 周进行悬尾实验(tail suspension test,TST)和强迫游泳实验(forced swimming test, FST)。第7 周行为学实验结束后,取小鼠脑组织制备匀浆,通过酶联免疫吸附法(enzymelinkedimmunosorbent assay, ELISA)测定脑源性神经营养因子(brain derived neurotrophic factor, BDNF)含量。结果1)成功构建CUMS 小鼠模型。第5 周模型组小鼠悬尾不动时间明显增加,差异有显著性( P < 0. 01),强迫游泳不动时间增加,差异有显著性( P < 0. 05)。(2)氟西汀组与模型对照组相比,悬尾实验不动时间明显缩短,差异有显著性( P < 0. 01),强迫游泳实验不动时间缩短,差异无显著性( P > 0. 05);音乐组与模型对照组相比,悬尾不动时间缩短,差异有显著性( P < 0. 05),强迫游泳实验不动时间无明显改变,差异无显著性( P > 0. 05)。模型对照组与空白组小鼠相比,脑组织匀浆中的BDNF 含量明显降低,差异有显著性( P < 0. 01);氟西汀组与模型对照组相比,脑组织匀浆中的BDNF 含量明显回升,差异有显著性( P < 0. 01),但音乐组与模型对照组相比,其差异无显著性( P >0. 05)。结论 莫扎特K448 奏鸣曲高频段声波可一定程度优化小鼠抑郁模型的治疗作用。
英文摘要:
      Objective A C57BL/6 mouse chronic unpredictable mild stress model of depression was establishedto investigate the effect of high frequency sound waves in Mozart’s K448 Sonata on depression. Methods Establishment ofa chronic stress model: Mice were divided into a blank group (n = 10) lived with no stress and model group (n = 36)established 5 weeks of chronic mild and unpredictable stimulation (CUMS). Therapeutic intervention: The mice in themodel group were randomly divided into the model control group (n =12), fluoxetine group (n =12), and music group (n= 12) after 5 weeks. Fluoxetine hydrochloride solution (10 mg/ kg) was injected intraperitoneally every day in thefluoxetine group, and the other two groups were injected with the same amount of saline lasted 2 weeks. The music groupreceived a 2-hour high frequency music intervention every day lasted 2 weeks, while the other two groups did not. Outcomevariables: Weight was recorded 3 days before the experiment and every week during the experiment. Tail suspension test(TST) and forced swimming test (FST) were performed in weeks 1, 5, and 7. At the end of week 7, mice were sacrificedand brain homogenates were prepared. BDNF levels were determined using an enzyme-linked immunosorbent assay(ELISA). Results At week 5, in the TST, immobility times of the model group were significantly longer than that of theblank group ( P < 0. 01). In the FST, immobility times of the model group were longer than that of the blank group ( P <0. 05). Compared with the model control group, both the fluoxetine group and music group exhibited a significantly shorterimmobility time of tail suspension ( P < 0. 01, P < 0. 05) Compared with the blank group, the model control group had asignificant lower BDNF content in brain homogenates ( P < 0. 01); compared with the model control group, the fluoxetinegroup had a significantly higher BDNF content ( P < 0. 01), and there was no significant difference in BDNF contentbetween the music group and the model control group ( P > 0. 05). Conclusion Mozart K448 Sonata high frequency sound waves may optimise the therapeutic effect on depression mice models.
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