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蒋学泉,张文云,李娜,何武书,和丽佳,袁艳波.树鼩背部肌肉植入多孔复合材料HAPw/ n-ZnO 的体内生物学性能[J].中国实验动物学报,2019,27(4):466~472.
树鼩背部肌肉植入多孔复合材料HAPw/ n-ZnO 的体内生物学性能
Biological properties of porous composite material HAPw / n-ZnO inplanted into the back muscle of tree shrew
投稿时间:2019-01-07  
DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 007
中文关键词:  HAPw/ n-ZnO 复合材料  异位成骨  生物活性  炎症反应  树鼩
英文关键词:HAPw/ n-ZnO porous composite material  ectopic osteogenesis  biological activity  inflammatory reaction  tree shrew
基金项目:
作者单位E-mail
蒋学泉 中国人民解放军联勤保障部队第九二〇医院口腔科 昆明 650032 352364866@ qq.com 
张文云 中国人民解放军联勤保障部队第九二〇医院口腔科 昆明 650032 wenyunzh88@ 126.com 
李娜 中国医学科学院/ 北京协和医学院医学生物学研究所树鼩种质资源中心,昆明 650118  
何武书 中国人民解放军联勤保障部队第九二〇医院口腔科 昆明 650032  
和丽佳 中国人民解放军联勤保障部队第九二〇医院口腔科 昆明 650032  
袁艳波 中国人民解放军联勤保障部队第九二〇医院口腔科 昆明 650032  
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中文摘要:
      目的 初步探讨多孔复合材料HAPw/ n-ZnO 的体内生物学性能。方法 选用树鼩15 只,雌雄不限,每只背部肌肉均植入多孔复合材料HAPw/ n-ZnO、Bio-Oss 骨粉、ATLANTIK 人工骨、国产金世植骨灵人工骨,多孔复合材料HAPw/ n-ZnO 为实验组,其余为对照组。术后进行动物大体观察及手术部位观察,4 周、8 周、12 周每次随机抽取4 只动物处死,进行埋植部位肌肉组织病理学观察、碱性磷酸酶活性(ALP)及钙含量测定。结果 HE 染色显示实验HAPw/ n-ZnO 组与各对照组肌间质内均可见钙化灶,实验组肌肉可见炎症细胞明显聚集;Masson 染色显示实验组与各对照组植入材料周边均可见绿染的胶原纤维;碱性磷酸酶活性及钙含量测定实验组与金世植骨灵组及Bio-Oss 骨粉组的差异有统计学意义,金世植骨灵组及Bio-Oss 骨粉组优于实验组,与ATLANTIK 人工骨组差异无统计学意义。结论 多孔复合材料HAPw/ n-ZnO 在植入树鼩背部肌肉后有成骨活性但未异位成骨,且引起炎症反应。
英文摘要:
      Objective  To explore the biological properties of the porous composite material HAPw/ n-ZnOimplanted into the back muscles of tree shrew. Methods Fifteen healthy 12-month old male and female tree shrews wereselected, and each of the back muscles were implanted with porous composite materials, including HAPw/ n-Zno, bio-ossbone powder, ATLANTIK artificial bone, and domestic product Jinshi Zhiguling artificial bone. Four animals were randomlysacrificed at 4 weeks, 8 weeks, and 12 weeks after surgery, and the gross observation, histopathological examination,alkaline phosphatase activity (ALP), and calcium content were determined. Results The pathological examination usingHE staining revealed calcification in the muscle tissues in both of HAPw/ n-ZnO and control groups. Obvious inflammatorycell infiltration in the muscle tissues was seen in the experimental group. Masson staining showed green-stained collagenfibers around the implanted material in both the experimental and control groups. There were significant differences in thealkaline phosphatase activities and calcium concentration among the experimental, Jinshi Zhiguling artificial bone, and Bio-Oss groups. The result of Jinshi Zhiguling artificial bone and Bio-Oss groups were better than the experimental group, andthere were no significant differences compared with that of ATLANTIK artificial bone group. Conclusions The four kindsof materials have osteogenic activity in the back muscle of tree shrews, with no heterotopic osteogenesis, and induce inflammatory reaction.
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