首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
谭启钊,牛国栋,赵振达,李思维,宋纯理,冷慧杰.关节软骨与软骨下骨改变在不同骨关节炎动物模型中的特点[J].中国实验动物学报,2019,27(4):450~455.
关节软骨与软骨下骨改变在不同骨关节炎动物模型中的特点
Alterations of articular cartilage and subchondral bone in different rat models of osteoarthritis
投稿时间:2019-01-17  
DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 005
中文关键词:  骨关节炎  骨关节炎动物模型  关节软骨  软骨下骨  骨小梁分析
英文关键词:osteoarthritis, OA  animal model  articular cartilage  subchondral bone  trabecular analysis
基金项目:
作者单位E-mail
谭启钊 北京大学第三医院骨科,北京 100191 tanqz@ bjmu.edu.cn 
牛国栋 北京脊柱疾病重点实验室,北京 100191  
赵振达 北京大学第三医院骨科,北京 100191  
李思维 北京大学第三医院骨科,北京 100191  
宋纯理 北京脊柱疾病重点实验室,北京 100191  
冷慧杰 北京大学第三医院骨科,北京 100191 lenghj@ bjmu.edu.cn 
摘要点击次数: 92
全文下载次数: 21
中文摘要:
      目的 探讨不同骨关节炎(osteoarthritis, OA)动物模型中软骨下骨和关节软骨的病理改变特征。方法 采用三种SD 大鼠骨关节炎模型,将24 只6 月龄雌性大鼠随机分为4 组:假手术对照组(Sham,n = 6),前交叉韧带切除手术组(ACLT,n =6),木瓜蛋白酶关节腔注射组(Papain,n =6),以及卵巢切除术组(OVX,n = 6)。造模后8 周取膝关节,胫骨平台行Micro-CT 扫描分析,关节软骨行甲苯胺蓝染色、Mankin 评分,比较软骨下骨和关节软骨改变情况。结果 造模操作后8 周,不同OA 模型的软骨破坏程度有所不同。ACLT 和Papain 组软骨破坏比较严重,OVX 组软骨变化较轻。所有OA 模型中的软骨下松质骨均发生改变,OVX 组相对于Sham 对照组,软骨下骨微结构显著疏松,而ACLT 组与Papain 组相对于Sham 对照组,软骨下骨微结构没有显著改变,但相对于OVX 组,有显著性差异。三种OA 模型的软骨下骨板厚度都较Sham 组减少。结论 三种动物模型软骨下骨和关节软骨都发生明显病理改变,并且改变有所不同。不同OA 模型代表不同病理,预示着软骨下骨所发挥的作用有所不同,这为进一步研究不同类型OA 发生发展的机制,以及将软骨下骨作为OA 治疗的可能靶点提供了更多的依据。
英文摘要:
      Objective To study the pathological changes of subchondral bone and cartilage in different osteoarthritis(OA) models. Methods Three Sprague Dawley (SD) OA models were used and twenty-four female rats were divided into fourgroups randomly: control group (Sham, n=6), anterior cruciate ligament transection operations group (ACLT, n =6), papainjoint cavity injection group (Papain, n=6) and ovariectomy group (OVX, n=6). After 8 weeks, all knee joints were extracted.Subchondral bone specimens from tibia plateau were scanned, and trabecular parameters were obtained. Articular cartilagespecimens were stained by toluidine blue, and OA was scored according to Mankin’s histological grading system. Results After8 weeks, the OA severity of cartilage are different for different OA models. Severe cartilage damage was observed in the ACLTand Papain groups. Only mild cartilage damage was occurred in the OVX group. Subchondral bone in all OA models also changedat week 8 post operation. Subchondral trabecular bone in the OVX group became looser than the Sham group. Subchondraltrabecular bone in the ACLT group and Papain group didn’t change significantly compared with the Sham group, but hadsignificant difference from the OVX group. Thickness of subchondral bone plate in all groups became thinner than the Shamgroup. Conclusions Both subchondral bone and cartilage changed with OA progress for all the three OA animal models. Thosealterations in different OA models are different, which implies that subchondral bone might play different roles for different typesof OA. This study will provide us more evidences to further investigate mechanisms of different types of OA and more insight to treat subchondral bone as an OA therapeutic target.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 1242563 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb1@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码