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刘军平,潘永明,陈民利,朱科燕,蔡月琴,吕涛,黄俊杰.白毛黑眼兔与日本大耳白兔胰岛素抵抗动脉粥样硬化模型的比较[J].中国实验动物学报,2019,27(3):339~346.
白毛黑眼兔与日本大耳白兔胰岛素抵抗动脉粥样硬化模型的比较
Comparison of the WHBE and Japanese white rabbit models of insulin resistance atherosclerosis
投稿时间:2018-12-03  
DOI:10. 3969 / j.issn.1005-4847. 2019. 03. 011
中文关键词:  白毛黑眼兔  胰岛素抵抗  动脉粥样硬化  高脂高糖饮食  氧化应激
英文关键词:white hair black eye rabbits  insulin resistance  atherosclerosis  high fat and sugar
基金项目:
作者单位E-mail
刘军平 浙江中医药大学动物实验研究中心,杭州 310053 liujp89day@ 126.com 
潘永明 浙江中医药大学动物实验研究中心,杭州 310053  
陈民利 浙江中医药大学动物实验研究中心,杭州 310053  
朱科燕 浙江中医药大学动物实验研究中心,杭州 310053  
蔡月琴 浙江中医药大学动物实验研究中心,杭州 310053  
吕涛 浙江中医药大学动物实验研究中心,杭州 310053  
黄俊杰 浙江中医药大学动物实验研究中心,杭州 310053 306999492@ qq.com 
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中文摘要:
      目的 探讨白毛黑眼(WHBE)兔和日本大耳白(JW)兔胰岛素抵抗动脉粥样硬化(insulin resistanceatherosclerosis, IR-AS)模型的表型差异和病理机制?方法 取WHBE 兔与JW 兔各12 只,分为正常对照组(NC)和高脂高糖饮食(HF)组,每组6 只?用HF 饮食12 周诱发IR-AS 模型?造模结束后,取血测定血脂?超氧化物歧化酶(SOD)和丙二醛(MDA)水平;行糖耐量试验,计算血糖和胰岛素曲线下面积;检测肝微粒体甘油三酯转运蛋白(MTTP)?核因子E2(Nrf2)和SOD1 基因的表达,并观察脂肪和主动脉血管的HE 染色病理变化以及血管CD68 的表达情况?结果 与NC 组比,HF 组肥胖,血脂升高,糖耐受不良,高胰岛素血症和胰岛素抵抗指数(HOMA-IR)明显升高,血浆及肝SOD 活性下降且MDA 含量升高,肝MTTP 和Nrf2 基因表达升高且SOD1 基因表达降低,血管脂质沉积和AS 以及血管CD68 表达显著升高;与JWHF 组比,WBHF 组TG?LDL-C?HOMA-IR?糖耐量曲线下面积(U_GLU)?MDA 含量?脂肪直径大小?肝SOD1 基因表达?AS 病变程度及血管CD68 表达上有明显差异?结论 高脂高糖饮食能诱导兔形成IR-AS,表现出脂代谢紊乱?炎症和AS 病变?但WHBE 兔的病变程度明显严重于JW 兔,这可能与这两种品系兔在脂质代谢和氧化应激反应存在差异有关?
英文摘要:
      Objective To explore the phenotypic differences and pathological mechanisms of insulin resistanceand atherosclerosis (IR-AS) in white hair black eye (WHBE) rabbits and Japanese white (JW) rabbits. Methods Twelve JW rabbits and 12 WHBE rabbits were randomly divided into the normal control group (NC) and high fat and sugardiet (HF) group with six rabbits in each group. The IR-AS model was induced by feeding a high fat and sugar diet for 12weeks. At the end of modeling, blood samples were collected to determine blood lipid, superoxide dismutase (SOD), andmalondialdehyde (MDA) levels. A glucose tolerance test was conducted, and the area under the curves of blood glucoseand insulin was calculated. mRNA expression of microsomal triglyceride transfer protein (MTTP), nuclear factor-like 2(Nrf2), and SOD1 genes in the liver was detected, pathological changes in fat tissues and aortic wall by HE staining, andCD68 expression in aortic tissues were observed. Results Compared with the NC group, HF rabbits showed significantobesity, hyperlipidemia, glucose intolerance, hyperinsulinemia, and increased HOMA-IR. Moreover, HF rabbits exhibiteddecreased SOD activity and increased MDA contents in their plasma and liver, increased mRNA expression of MTTP andNrf2 in the liver tissue, and decreased mRNA expression of SOD1. In addition, vascular lipid deposition, AS plaque size,and CD68 expression were increased significantly. Compared with the JWHF group, TG, LDL-C, HOMA-IR, U_GLU,MDA content, fat diameter, SOD1, AS lesion degree, and vascular CD68 expression in the WBHF group were significantlydifferent. Conclusions A high-fat and sugar diet induces the formation of IR-AS in rabbits, resultsing in an obvious lipidmetabolism impairment, inflammatory and AS lesions. However, the degree of pathological changes in WHBE rabbits issignificantly more serious than that in JW rabbits, which may be related to the differences in lipid metabolism and the oxidative stress response between the two rabbit strains.
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