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李振宗,薛亚轲,高华.微弧氧化和碱处理技术在多孔钽修复兔颅骨缺损中的应用[J].中国实验动物学报,2019,27(3):316~322.
微弧氧化和碱处理技术在多孔钽修复兔颅骨缺损中的应用
Application of micro-arc oxidation and NaOH treatment in the repair of rabbit skull defect using bioactive tantalum metal
投稿时间:2019-03-17  
DOI:10. 3969 / j.issn.1005-4847. 2019. 03. 008
中文关键词:  多孔钽  骨修复  生物相容性  微弧氧化  碱修复  
英文关键词:porous tantalum  skull repair  biocompatibility  micro-arc oxidation  alkali treatment  rabbit
基金项目:
作者单位E-mail
李振宗 北京市神经外科研究所,北京 100070 13811971870@ 163.com 
薛亚轲 首都医科大学附属北京天坛医院,北京 100070  
高华 中枢神经系统损伤研究北京市重点实验室,北京100070 huagao@ aliyun.com 
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中文摘要:
      目的 观察微弧氧化和碱处理对多孔钽表面性状?生物相容性和成骨能力的影响?方法 微弧氧化和碱处理多孔钽片后,扫描电镜观察表面微孔数量?表面钙磷沉积和接触角?植入钽片修复兔颅骨缺损模型,在4 周和12 周观察骨愈合情况?结果 扫描电镜显示处理组表面有更多的微孔和钙磷沉积以及更小的接触角( P <0. 05)?植入多孔钽片后,所有动物均生长良好,伤口愈合佳?CT 观察多孔钽片和周围骨组织耦合良好;钙黄绿素标记检测显示12 周时有新生骨长入多空钽材料内部;扫描电镜观察发现4 周时多空钽材料内部有新生血管,12 周时有骨小梁长入材料内部?结论 微弧氧化和碱处理能改变多孔钽材料表面形状,处理后多孔钽片具有良好的生物相容性和成骨能力?
英文摘要:
      Objective To observe the effects of micro-arc oxidation (MAO) and alkali (NaOH) treatment on theporous surface tantalum, and evaluate the biocompatibility and osteogenic ability of porous tantalum in vivo. Methods Porous tantalum was immersed in simulated body fluid (SBF) for 1 week after MAO and alkali treatment. Micropores,calcium, and phosphorus deposition on the surface were observed by scanning electron microscopy (SEM). The contact anglewas observed by X-ray photoelectron spectroscopy (XPS). For in vivo experiments, bone healing was evaluated at 4 and 12weeks after the porous tantalum implantation into a rabbit skull defect model. Results Compared with the control and MAOonlytreated specimens, NaOH-treated tantalum metal formed more apatite on its surface in SBF. Moreover, a sodium tantalatehydrogel layer and reduced contact angle were observed on the specimen surface by XPS ( P < 0. 05). In vivo, the woundshealed well and there was no swelling or suppuration in the rabbit implant model. Computerized tomography imaging revealedthat the porous bract and surrounding bone tissue were well coupled after 4 and 12 weeks. Calcein fluorescent labeling anddetection indicated that new bone had grown into the interior of the multi-porous material at 12 weeks. Moreover,neovascularization and new trabecular bone were observed by SEM. Conclusions MAO and alkali treatment can change the surface shape of porous tantalum material, exhibiting good biocompatibility and osteogenic ability in vivo.
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