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鞠静,彭玲玲,潘宇政,谭人千,潘茂华,韦进新.淫羊藿苷及宝藿苷I 对气管切开模型大鼠肺组织病理学及炎症因子的影响[J].中国实验动物学报,2019,27(3):305~310.
淫羊藿苷及宝藿苷I 对气管切开模型大鼠肺组织病理学及炎症因子的影响
Effects of icariin and baohuoside I on the pathological changes and inflammatory factors in tracheotomized rats
投稿时间:2018-11-15  
DOI:10. 3969 / j.issn.1005-4847. 2019. 03. 006
中文关键词:  淫羊藿苷  宝藿苷I  气管切开  组织病理学  白细胞介素6  肿瘤坏死因子α    大鼠
英文关键词:icariin  baohuoside I  tracheotomy  histopathology  IL-6  TNF-α  lung  rat
基金项目:
作者单位E-mail
鞠静 广西医科大学第一附属医院, 南宁 530021 henanju6220@ 163.com 
彭玲玲 广西医科大学第一附属医院, 南宁 530021  
潘宇政 广西医科大学第一附属医院, 南宁 530021 pyz79298@ sina.com 
谭人千 广西医科大学第一附属医院, 南宁 530021  
潘茂华 广西上林县人民医院,南宁 530500  
韦进新 南宁市第四人民医院,南宁 530021  
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中文摘要:
      目的 探讨淫羊藿苷及其代谢产物宝藿苷I 对气管切开模型大鼠肺组织病理及炎症的影响?方法 将72 只SPF 级雄性SD 大鼠随机分成四组,正常对照组(A)?模型非用药组(B)?模型+宝藿苷I 组(C)?模型+淫羊藿苷组(D),每组18 只?制备大鼠气管切开插管留置模型?模型成功6 h 后给予C 组宝藿苷I,D 组淫羊藿苷干预,A?B 组给与等量0. 9% 生理盐水?分别在24?72?168 h 取肺组织及肺泡灌洗液?肺组织用苏木精-伊红染色(HE 染色),光镜下观察分析肺组织的病理学改变;ELISA 检测肺泡灌洗液白细胞介素6(IL-6),肿瘤坏死因子α(TNF-α)表达?结果 1)肺组织病理结果显示:气管切开后B 组大鼠肺损伤程度随时间延长逐渐加重,明显高于A 组( P < 0. 05)?C?D 组肺损伤程度均明显轻于B 组,且D 组在24?168 h 均明显轻于C 组( P < 0. 05)?D 组在168h 病理评分明显降低(与24 h 比较 P < 0. 01)?(2)肺泡灌洗液IL-6?TNF-α 含量:气管切开插管后大鼠肺泡灌洗液IL-6 和TNF-α 含量在三个时段均明显高于A 组( P < 0. 05)?用宝藿苷I 和淫羊藿苷干预后,C?D 组IL-6?TNF-α 含量在72?168 h 明显低于B 组( P < 0. 05);且C?D 组组内IL-6 和TNF-α 含量在72?168 h 明显低于24 h( P < 0. 05);D组与C 组相比,IL-6 含量无明显统计学差异( P > 0. 05)?D 组TNF-α 在72 h?168 h 均明显低于C( P < 0. 05)?结论 宝藿苷I 和淫羊藿苷对气管切开术后早期肺部炎症有一定疗效,其机制与抑制肺泡灌洗液炎症因子IL-6?TNF-α 的表达,减轻肺组织损伤有关,且淫羊藿苷效果更佳?
英文摘要:
      Objective To investigate the effects of icariin and its metabolite, baohuoside I, on the pathology andinflammation of lung tissue in tracheotomized rats. Methods Seventy-two SPF male Sprague-Dawley rats were randomlydivided into four groups: normal control group (A), model control group (B), and model treatment group (C and D) with18 rats in each group. A rat tracheotomy tube indwelling model was established. At 6 hours after successful modeling, thegroups C and D were administered icariin and baohuoside I, respectively. The groups A and B were administered an equalamount of 0. 9% normal saline. Lung tissue and alveolar lavage fluid were collected at 24, 72, and 168 h. Pathologicalexamination of the lung tissues was performed using HE staining. ELISA was used to detect the interleukin-6 (IL-6) andtumor necrosis factor alpha (TNF-α) in alveolar lavage fluid. Results After the tracheotomy, the degree of histologicalchanges in the lung tissues of the group B was gradually increased over time, significantly higher than that in the group A( P < 0. 05). The degree of lung injury in the groups C and D was significantly lower than that in the group B, and that inthe group D was significantly lower compared with the group C at 24 and 168 h after tracheotomy ( P < 0. 05). Thepathological score of group D was significantly lower at 168 h ( P < 0. 01 compared with 24 h). The contents of IL-6 andTNF-α in alveolar lavage fluid of the rats after tracheotomy were significantly higher than those in the group A ( P < 0. 05).After treatments with baohuoside I and icariin, the levels of IL-6 and TNF-α in the groups C and D were significantly lowerthan those in the group B at 72 and 168 h ( P <0. 05), and the IL-6 and TNF-α contents in the groups C and D at 72 h and168 h were significantly lower than that after 24 h ( P <0. 05). There was no significant difference in the IL-6 contents ofgroups C and D at 72 h and 168 h ( P > 0. 05). TNF-α contents in the group D at 72 and 168 h were significantly lowerthan that in the group C ( P < 0. 05). Conclusions Baohuoside I and icariin have certain therapeutic effects on the earlylung inflammation after tracheotomy. The mechanism is related to inhibiting the release of inflammatory factors IL-6 andTNF-α into alveolar lavage fluid and alleviating lung tissue damage. Moreover, the effect of icariin is better than that of baohuoside I.
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