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付超,常静林,姜轩,张从柔,张玉民,褚丽萍,赵瑞利.响应性聚集金纳米粒子体系用于细菌的体外热疗研究[J].中国实验动物学报,2019,27(3):278~285.
响应性聚集金纳米粒子体系用于细菌的体外热疗研究
Responsive aggregation of gold nanoparticles system for photothermal therapy of bacteria in vitro
投稿时间:2018-12-19  
DOI:10. 3969 / j.issn.1005-4847. 2019. 03. 002
中文关键词:  金纳米粒子  响应性聚集  细菌  光热治疗
英文关键词:gold nanoparticles  responsive aggregation  bacteria  photothermal therapy
基金项目:
作者单位E-mail
付超 1. 天津农学院动物科学与动物医学学院,天津 300384
2. 中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室,天津 300192 
18393813292@ 163.com 
常静林 中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室,天津 300192  
姜轩 天津农学院动物科学与动物医学学院,天津 300384  
张从柔 中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室,天津 300192  
张玉民 中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室,天津 300192  
褚丽萍 中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室,天津 300192  
赵瑞利 天津农学院动物科学与动物医学学院,天津 300384 zhaoruili1109@ 126.com 
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中文摘要:
      目的 利用发生响应性聚集后的金纳米粒子体系的较强的光热特性,研究其对细菌的体外光热杀伤作用?方法 通过Au-S 键反应将合成好的多肽A 和多肽B 分别修饰到金纳米粒子(GNPs)表面,然后等比例混合组成GNPs system?首先利用动态光散射(DLS)和透射电镜(TEM)研究其在弱酸性条件下的响应性聚集情况,利用多功能酶标仪研究其在在弱酸性条件下的紫外吸收变化;然后为了了解该纳米粒子在菌液中的光热转换情况,分别测定其在弱酸性条件下溶液内和与细菌作用后的温度变化曲线;进一步考察其体外抗菌效果?结果 DLS检测到合成的GNPs system 在弱酸性条件下粒径由16 nm 增大到900 nm 左右,并在TEM 下可见明显的聚集体,并且在650~900 nm 的紫外吸收信号明显增高?在模拟细菌的弱酸性环境下,GNPs system 在激光照射条件下均实现了溶液和细菌混合溶液的快速升温,且最高温度可达69. 8℃,与对照组GNPs-PEG2000 相比具有显著的统计学差异;体外抗菌实验结果显示,GNPs system 对金黄色葡萄球菌的杀伤力最强,50 μg/ mL 浓度时就可杀死约50%的细菌;浓度为200 μg/ mL 时基本上可以完全杀死,与对照组GNPs-PEG2000 相比较具有显著的统计学差异?结论 本研究为GNPs 的设计提供了新的思路,为GNPs 用于光热治疗提供了新的方法?
英文摘要:
      Objective To study the photothermal killing effect on bacteria means of the strong photothermalproperties of gold nanoparticles system (GNPs system) after responsive aggregation. Methods Synthesized peptides A andB were modified at their surface by gold nanoparticles (GNPs) through the Au-S bond reaction, and then mixed at equal Acta Lab Anim Sci Sin,June 2019,Vol. 27, No. 3proportions to form the GNPs system. First, dynamic light scattering (DLS) and transmission electron microscopy (TEM)were used to assess the responsive aggregation under weakly acidic conditions. UV absorption changes under weakly acidicconditions were measured by a multifunction microplate reader. In order to understand the photothermal conversion of thenanoparticles in the bacterial suspension, their temperature curves in the solution under weakly acidic conditions and aftertreated with bacteria were measured, respectively. Finally, the antibacterial effect was tested in vitro. Results The particlesize of the synthesized GNPs system was increased from 16 nm to 900 nm as detected by DLS under weakly acidic conditions.Obvious aggregates were observed by TEM, and the UV absorption signal was significantly increased at 650~900 nm. Underthe weakly acidic conditions of simulated bacteria, the GNPs system achieved a rapid temperature rise of the solution withmixed bacterial under laser irradiation conditions. The highest temperature was 69. 8°C, significantly different from the controlgroup of GNPs-PEG2000. The result of antibacterial experiments in vitro showed that the GNP system had the strongest killingeffect against Staphylococcus aureus, which was 50% and 100% killing of the bacteria at concentration of 50 and 200 μg/ mL,respectively, showing a significant difference compared with the control group of GNPs-PEG2000. Conclusions This study provides a new approach for the design of GNPs and a new method to apply GNPs to photothermal therapy.
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