血栓调节蛋白在大鼠深静脉血栓模型中的表达及其意义
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(1. 南通大学医学院,南通 226001; 2. 南通大学附属医院妇产科,南通 226001)

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Q95-33

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Expression and significance of thrombomodulin in a rat model of deep-vein thrombosis
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(1. Medical College of Nantong University, Nantong 226001, China. 2. Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226001)

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    摘要:

    目的 观察大鼠深静脉血栓(DVT)形成及消退的自然演变过程中血栓调节蛋白(TM)的表达及意义?方法 将SD 大鼠随机分为模型组(n =60)和对照组(n =6),模型组采用“狭窄法”建立DVT 模型,对照组采用“假手术法”?建模后分别于1 h?4 h?6 h?12 h?24 h?3 d?7 d?14 d?21 d?28 d 共10 个时间点处死?检测大鼠血栓重量/ 长度比值?血浆中可溶性TM 浓度?血栓组织及下腔静脉内皮中TM mRNA 的表达?结果 1)血栓重量/ 长度比值在造模早期(1?4?6?12 h)较低,四组间比较差异无显著性( P > 0. 05);在造模后24 h?3 d?7 d 稳定在一个较高的水平( P > 0. 05);造模后14 d 开始降低,但其与21?28 d 结果差异无显著性( P > 0. 05)?(2)ELISA 结果显示,血浆中可溶性TM 均较对照组高,差异有显著性( P < 0. 01),随时间推移总体逐渐升高?(3)实时荧光定量PCR 结果显示,血栓TM mRNA 表达随时间推移总体呈逐渐升高的趋势?血管内皮TM mRNA 表达在1?4?6?12 h 较对照组高( P < 0. 01);在24 h?3 d?7 d 较对照组低( P < 0. 01);14?21?28 d 与对照组相比,差异无显著性( P > 0. 05)?(4)血栓重量/ 长度比值和内皮TM mRNA 呈负相关(r=-0. 92, P < 0. 01);而血栓TM mRNA 和血浆可溶性TM 呈正相关(r=0. 96, P < 0. 01)?结论 内皮TM mRNA 的表达变化可以反映DVT 的病情进展,具有良好的预后作用?

    Abstract:

    Objective To observe the expression and explore the significance of thrombomodulin(TM) in theformation and regression of deep vein thrombosis (DVT) in rats. Methods Sprague-Dawley rats were divided randomly intotwo groups: model (n = 60) and control (n = 6) groups. A DVT model was established by stenosis (“stenosis method”)in the model group and a sham operation was performed in the control group. The rats were killed at 1, 4, 6, 12, 24 h aswell as 3, 7, 14, 21 and 28 days after modeling. The weight:length ratio of thrombi, plasma concentration of solubleTM, expression of TM mRNA in the thrombi and inferior vena cava (IVC) endothelium were measured. Results The weight:length ratio of thrombi was low in the early stage of modeling (1, 4, 6, 12 h), showing no significant differenceamong the four subgroups ( P > 0. 05). The model was stable at a higher level 24 h, 3 days and 7 days after modeling ( P >0. 05). It began to decrease 14 days after modeling, but there was no significant difference among the results at 14, 21 or28 days ( P > 0. 05). The ELISA showed that the concentration of soluble TM was significantly higher than that in thecontrol group ( P < 0. 01), and that the level of soluble TM in plasma increased gradually. The real-time fluorescencequantitative polymerase chain reaction showed that the mRNA expression of TM in thrombi increased gradually over time.Expression of TM mRNA in IVC endothelium was significantly higher at 1, 4, 6 and 12 h than that in the control group ( P < 0. 01) and significantly lower than that in the control group at 24 h, 3 days and 7 days ( P < 0. 01). There was nosignificant difference in expression of TM mRNA at 14, 21, or 28 days compared with that in the control group ( P >0. 05). A negative correlation between the weight:length ratio of thrombi and expression of TM mRNA in the IVCendothelium was determined (r = - 0. 92, P < 0. 01). However, expression of TM mRNA in thrombi was positivelycorrelated with the levels of soluble TM in plasma (r= 0. 96, P < 0. 01). Conclusions Our findings suggest that TM isinvolved in the entire process of the formation and regression of thromb, and the changes in endothelial TM expression mayreflect the occurrence and development of deep vein thrombosis,and is of a good prognostic significance.

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成曦,孙宝兰,苏张瑶,张玉泉.血栓调节蛋白在大鼠深静脉血栓模型中的表达及其意义[J].中国实验动物学报,2019,27(2):193~199.

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  • 收稿日期:2018-11-21
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  • 在线发布日期: 2019-05-05
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