Objective To generate a transgenic zebrafish line expressing growth hormone (GH) and evaluate theeffects of growth hormone (GH) overexpression on zebrafish caudal fin regeneration. Methods An expression plasmid(pDestTol2CG2; ubi:GH-polyA) was constructed by Gateway technology. After coinjection of the expression plasmid andtransposase-encoding mRNA at the one cell stage, GH-overexpressing transgenic zebrafish were screened by fluorescencemicroscopy and qPCR. The zebrafish were divided into wild type control and GH overexpression groups. After the zebrafishcaudal fin was transected, its regeneration process was recorded and analyzed. Results The heart of the transgeniczebrafish was labeled with green fluorescent protein. The real-time PCR showed that the expression level of GH wassignificantly higher than that in the wild type zebrafish ( P < 0. 05). After the zebrafish caudal fin was transected, theregeneration rate of the GH overexpression group was increased significantly ( P < 0. 05). Conclusions In this study, atransgenic zebrafish strain overexpressing GH is generated, and it demonstrates that GH overexpression promotes the regeneration of zebrafish caudal fin.