一种新型高脂血症易感大鼠的血脂水平与心血管并发症
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(浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053)

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Q95-33

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Blood lipid levels and cardiovascular complications in a new type of hyperlipidemia-susceptible rats
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(Animal Experimental Research Center/ Institute of Comparative Medicine, Zhejiang Chinese Medical University,Hangzhou 310053, China)

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    摘要:

    目的 观察一种新型高脂血症易感WSHc 大鼠经高脂饲料诱导后血脂的动态变化和心血管并发症病理特点,为WSHc 大鼠的应用提供参考。方法 取7~8 周龄雌性WSHc 大鼠20 只和Wistar 大鼠10 只,进行高脂饲喂,另取同周龄同性别的WSHc 大鼠和Wistar 大鼠各10 只饲喂普通饲料,分别作为正常对照。观察高脂饲喂2、4、8、12、16 周时的血脂动态变化,饲喂16 周后采用心动超声检测大鼠心功能及左心结构,病理组织学观察心脏及主动脉病变。结果 与正常对照组比,高脂饲喂2 周后WSHc 大鼠与Wistar 大鼠血清总胆固醇(TC)与低密度脂蛋白胆固醇(LDL-c)均显著升高;高脂饲喂的WSHc 大鼠TC、LDL-c 水平为:(6. 34±2. 12)mmol/ L、(2. 56±0. 94)mmol/ L,而高脂饲喂的Wistar 大鼠TC、LDL-c 水平仅为(2. 93±0. 23)mmol/ L,(0. 63±0. 12)mmol/ L;在高脂饲喂期间,WSHc 大鼠TC 始终维持在高水平状态。饲喂16 周后,WSHc 大鼠心脏射血分数升高,左心室室壁增厚,并出现心肌纤维化,心肌细胞凋亡程度增高;主动脉内膜增厚,弹力纤维排列紊乱,形成早期动脉粥样硬化病变。而Wistar大鼠经高脂饲料诱导后,心功能及心血管病变不明显。结论 WSHc 大鼠对外源性胆固醇敏感,高脂饲喂后易发高脂血症及心血管疾病,且TC、LDL-c 水平与临床高脂血症更接近,相较于普通大鼠,更适用于高脂血症及相关心血管并发症的实验研究。

    Abstract:

    Objective This study aimed to observe the dynamic variation of blood lipids and the pathological features of cardiovascular complications induced by a high-fat diet in a new type of hyperlipidemia-susceptible (WSHc)rats, and to provide a reference for the application of WSHc rats. Methods Twenty female WSHc rats and 10 Wistar rats,aged 7-8 weeks, were fed with a high-fat diet. Another 10 WSHc and Wistar rats of the same age and sex were given a normal diet as a control group. Dynamic changes of blood lipids in the rats were observed at 2, 4, 8,12, and 16 weeks.After feeding for 16 weeks, the cardiac function of the rats in each group was tested using a cardiac ultrasound, and pathological changes of the heart and aorta were observed by histopathology. Results After 2 weeks of the high-fat diet feeding, the serum total cholesterol (TC) of WSHc and Wistar rats was significantly higher than that of the control group.The levels of TC and LDL-c in high-fat-diet-fed WSHc rats were (6. 34±2. 12) and (2. 56±0. 94) mmol/ L, while the levels in the Wistar rats fed with high fat diet were only (2. 93±0. 23) and (0. 63±0. 12) mmol/ L, respectively; during high-fat diet feeding, the TC of WSHc rats remained at a high level. After 16 weeks of high-fat diet intervention, the cardiac ejection fraction of the WSHc rats was increased. Furthermore, the left ventricular wall was thickened, and myocardial fibrosis occurred. Additionally, the aortic intima was thickened, and the arrangement of elastic fibers was disordered, forming early atherosclerotic lesions. However, lesions and cardiovascular disease were not apparent in the Wistar rats after the high-fat diet. Conclusions WSHc rats are sensitive to exogenous cholesterol, and are prone to hyperlipidemia and related cardiovascular complications after high-fat diet feeding. Additionally, the TC and LDL-c levels are close to those in clinical hyperlipidemia. Compared with normal rats, they are more suitable for studying hyperlipidemia and related cardiovascular complications.

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马全鑫,陈姣姣,郁晨,张利棕,寿旗扬,陈民利.一种新型高脂血症易感大鼠的血脂水平与心血管并发症[J].中国实验动物学报,2019,27(1):25~31.

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  • 收稿日期:2018-09-17
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  • 在线发布日期: 2019-03-06
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