布比卡因致心肌细胞线粒体损伤大鼠模型的建立
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(1. 河北北方学院附属第一医院麻醉科,河北张家口 075000; 2. 河北省人民医院麻醉科,石家庄 050000;3. 邯郸市中心医院麻醉科,河北邯郸 056300; 4. 河北医科大学第三医院,石家庄 050000)

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Q95-33

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Establishment of a rat model of myocardial mitochondrial damage induced by bupivacaine
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(1. the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China. 2. Hebei General Hospital, Shijiazhuang 050000. 3. HanDan Central Hospital, Handan 056300. 4. the Third Hospital of Hebei Medical University, Shijiazhuang 050000)

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    摘要:

    目的 观察电刺激下布比卡因心肌细胞线粒体形态变化及活性氧(reactive oxygen species, ROS)生成量,探讨建立理想的布比卡因中毒的大鼠心肌细胞模型。方法 采用Langendroff 装置新鲜分离雄性SD 大鼠心肌细胞,细胞计数后将其转移至doff 管中随机分为四组:DMEM 静置组、DMEM 电刺激组、布比卡因静置组、布比卡因电刺激组。实验重复五次。采用透射电镜观察心肌细胞线粒体形态,并使用多功能微孔板检测仪测量ROS 生成量。结果 DMEM 电刺激组线粒体肿胀程度及ROS 生成量与DMEM 静置组相比差异无显著性( P > 0. 05);而布比卡因电刺激组线粒体肿胀程度明显高于布比卡因静置组( P = 0. 000),且ROS 生成量也明显升高( P < 0. 05)。结论 电刺激下心肌细胞呈节律性收缩,能更好地模拟临床布比卡因中毒时心肌线粒体损伤。

    Abstract:

    Objective To observe the morphological changes of cardiomyocytic mitochondria and the reactive oxygen species content in bupivacaine-exposed myocardial cells under electrical stimulation, and to establish an ideal myocardial cell model of bupivacaine poisoning. Methods A Langendorff device was used to separate the cardiomyocytes of male SD rats. The cells were divided into four groups randomly: a DMEM static group, DMEM electric stimulation group, bupivacaine static group and bupivacaine plus electric stimulation group. The experiment was repeated for five times.The cardiomyocytic mitochondrial morphology was observed by transmission electron microscopy, and the ROS content was measured with a multifunctional microplate detector. Results The degree of mitochondrial swelling and the ROS content in the DMEM electric stimulation group were not significantly different from those of the DMEM group ( P > 0. 05), but the mitochondrial swelling in the bupivacaine plus electric stimulation group was significantly higher than that of the bupivacaine group ( P =0. 000), and the ROS output was also significantly increased ( P < 0. 05). Conclusions Under electrical stimulation, cardiomyocytes show rhythmic contractions, allowing better simulation of the myocardial mitochondrial injury during clinical bupivacaine poisoning.

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张颖颖,段然,陈永学,赵森明,夏登云.布比卡因致心肌细胞线粒体损伤大鼠模型的建立[J].中国实验动物学报,2019,27(1):13~18.

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  • 收稿日期:2018-07-25
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  • 在线发布日期: 2019-03-06
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