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任全,陈旭辉,陈明,杨建军.组合行为学实验方法检测东莨菪碱制作小鼠谵妄模型[J].中国实验动物学报,2018,26(5):644~648.
组合行为学实验方法检测东莨菪碱制作小鼠谵妄模型
Combined behavioral test to detect delirium induced by scopolamine in mice
投稿时间:2018-04-11  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 05. 017
中文关键词:  谵妄  小鼠  黑白箱  旷场实验  注意力水平  东莨菪碱
英文关键词:delirium  mice  light and black box test  open?field test  non?selective non?sustained attention test  scopolamine
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作者单位E-mail
任全 东南大学附属中大医院麻醉科,南京 210009 g_quanren@ yahoo. com 
陈旭辉 华中科技大学同济医学院附属同济医院麻醉科,武汉 430030  
陈明 东南大学附属中大医院泌尿科,南京 210009  
杨建军 东南大学附属中大医院麻醉科,南京 210009  
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中文摘要:
      目的 用组合行为学实验方法检测东莨菪碱制作小鼠谵妄模型?方法 比较两组小鼠行为学变化:谵妄组(n =12)和对照组(n =8)小鼠分别腹腔注射15 mg/ kg 东莨菪碱和等容量的生理盐水,在注射后30 ~60min 之间对两组小鼠顺序进行黑白箱实验10 min,旷场实验10 min 和非选择性非持续性注意力实验10 min—组合行为实验法,比较两组小鼠的5 项行为学指标以检测谵妄状态?结果 与对照组相比,谵妄组小鼠5 项行为学指标差异均有显著性,小鼠在白箱中停留的时间显著缩短, P < 0. 05;小鼠的活动速度显著增快, P < 0. 05,在沿壁区活动时间显著增多, P < 0. 05,僵住时间显著减少, P < 0. 05;小鼠“注意力”水平显著下降, P < 0. 05?结论 组合行为实验法能反映谵妄诊断要素,更好地反映谵妄状态,且用时较短?
英文摘要:
      Objective To develop a combined behavioral test for assessment of scopolamine?induced delirium in mice. Methods Two groups of mice were randomly assigned to receive 15 mg/ kg scopolamine (delirium group; n =12) or isovolemic saline (control group; n = 8) via intraperitoneal injection. We developed a novel behavioral experiment in which mice received the light and black box test for 10 min, the open?field test for 10 min, and then the non?selective non?sustained attention test for 10 min, from 30 to 60 min after intervention. Five behavioral indexes were compared between the groups to assess the delirium status. Results Compared with the control group, there were significant differences in all five behavioral indexes in mice in the delirium group mice at 30 min after intervention: time spent in the white box was significantly shorter ( P < 0. 05), the movement speeds were significantly increased ( P < 0. 05), time spent beside the wall area was increased significantly ( P < 0. 05), the freezing time, and the attention level were significantly decreased ( P < 0. 05). Conclusions This combined behavioral experiment can be used to assess the key elements for delirium diagnosis, and with a faster time than conventional tests.
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