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张蕊,易月娥,杜牧,赵蕾,张惠铭,左从林.老龄大鼠自发性肝胆管增生的病理学研究[J].中国实验动物学报,2018,26(5):561~566.
老龄大鼠自发性肝胆管增生的病理学研究
Pathological examination of spontaneous liver bile duct hyperplasia in aging rats
投稿时间:2018-04-20  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 05. 004
中文关键词:  老龄大鼠  自发性胆管增生  病理学    卵圆细胞  SD 大鼠  Wistar 大鼠
英文关键词:aging rat  spontaneous bile duct hyperplasia  pathologist  liver  oval cell  Sprague Dawley rats  Wistar rats
基金项目:
作者单位E-mail
张蕊 北京昭衍新药研究中心,北京 100176 zhangrui@ joinn?lab. com 
易月娥 北京昭衍新药研究中心,北京 100176  
杜牧 北京昭衍新药研究中心,北京 100176  
赵蕾 北京昭衍新药研究中心,北京 100176  
张惠铭 北京昭衍新药研究中心,北京 100176  
左从林 北京昭衍新药研究中心,北京 100176 zuocl@ joinn?lab. com 
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中文摘要:
      目的 为相关科研及新药安全评价工作积累大鼠肝胆管增生有价值的研究资料?方法 大鼠共分为3 组,第1 组雌雄各30 只动物(进口SD 大鼠);第2 组雌雄各60 只(国产SD 大鼠);第3 组雌雄各60 只(国产Wistar 大鼠)?实验末期,对所有实验动物进行安乐死,进行系统解剖,对肝进行制片,进行组织病理学检查和免疫组织化学研究?结果 各组大鼠均发生了不同程度的肝汇管区胆管增生,总发病率是32. 33%?其中国产SD 大鼠发病率明显高于进口SD 大鼠(26. 67% ∶ 1. 67%);国产Wistar 大鼠的发生率明显高于国产SD 大鼠(53. 33% ∶26. 67%);雄性动物的发病率明显高于雌性动物(20%∶ 12. 33%)?病理学观察显示多样化的胆管增生和纤维化改变,病变在I 级和II 级的大鼠发病率是84. 5%,III 级病变的发病率仅占15. 5%?卵圆细胞的增生与胆管增生病变情况相一致,并呈现向胆管上皮方向分化?结论 不同种系?不同性别的大鼠间肝胆管增生的发生率存在差异?本研究结果为动物和人类在增龄情况下肝胆管增生的研究提供了参考资料?
英文摘要:
      Objective To examine the incidence and pathological characteristics of spontaneous liver bile duct hyperplasia in aging rats. Methods Rats were divided into three groups: Group 1, 30 imported Sprague Dawley (SD) male rats and 30 imported SD females; Group 2, 60 domestic SD males and 60 domestic SD females; and Group 3, 60 domestic Wistar males and 60 domestic Wistar females. The animals were euthanized after feeding for 104 weeks. The livers were collected for conventional histopathological and immunohistochemical analyses. Results Bile duct hyperplasia was observed in all groups, with an overall incidence rate of 32. 33%. However, the incidence rate of bile duct hyperplasia in domestic SD rats was higher than that in the imported SD rats (26. 67% vs 1. 67%, respectively), the incidence rate was higher in domestic Wistar rats than that in domestic SD rats (53. 33% vs 26. 67%, respectively), and the incidence rate was higher in the male rats than that in the female rats (20% vs 12. 33%, respectively). Multiple morphological bile duct hyperplasia findings with fibrosis were seen by pathological examination. We defined three hyperplasia grades to evaluate the pathological lesions. The incidence rate of lesions in grade I and grade II was 84. 5%, while the incidence of lesions in grade III was 15. 5%. Hyperplasia of oval cells was observed in cases with bile duct hyperplasia, with differentiation to bile duct epithelium. Conclusions The incidence rate of liver bile duct hyperplasia in aging rats varies by species and sex. These data provide a reference for studies on liver bile duct hyperplasia in ageing animals and humans.
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