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陈吉莉,袁盛梦,陈芳园,陈荟宇,韩沛丰,王福.化学灼烧法大鼠口腔溃疡动物模型对照优化的研究[J].中国实验动物学报,2018,26(5):554~560.
化学灼烧法大鼠口腔溃疡动物模型对照优化的研究
Optimization of the establishment chemically?induced of rat model of oral ulcer
投稿时间:2018-05-15  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 05. 003
中文关键词:  口腔溃疡  化学灼烧法  SD 大鼠  动物模型
英文关键词:oral ulcer  chemical burning  Sprague Dawley rats  animal model
基金项目:
作者单位E-mail
陈吉莉 大连医科大学口腔医学院,大连 116044 544519065@ qq. com 
袁盛梦 大连医科大学口腔医学院,大连 116044 1243357405@ qq. com 
陈芳园 大连医科大学口腔医学院,大连 116044  
陈荟宇 大连医科大学口腔医学院,大连 116044  
韩沛丰 大连医科大学口腔医学院,大连 116044  
王福 大连医科大学口腔医学院,大连 116044 dywangfu@163. com 
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中文摘要:
      目的 通过不同化学灼烧法建立SD 大鼠口腔溃疡动物模型,为治疗效果评价筛选最优方案?方法 将50 只SD 大鼠随机分为5 组?采用不同浓度冰醋酸或NaOH 晶体于下切牙牙槽骨唇侧黏膜和颊黏膜建立溃疡模型;二次灼烧组:采用50%浓度冰醋酸于牙槽骨唇侧黏膜溃疡愈合后原部位再次建立溃疡模型?观察黏膜状态?溃疡出现率和愈合时间及组织病理学改变?结果 牙槽骨唇侧黏膜50% 浓度冰醋酸组溃疡出现率高,形态规则便于观察,平均愈合时间和组织学特征与人类接近,综合比较优于其他组?二次灼烧组不易形成溃疡?结论 50%浓度冰醋酸处理牙槽骨唇侧黏膜能获得较好的口腔溃疡模型,可作为研究溃疡治疗方法的理想模型?同一部位不适合再次建立溃疡模型?
英文摘要:
      Objective To determine the optimal technique for establishing a chemically?induced oral ulcer model in rats for future treatment studies. Methods A total of 50 Sprague Dawley rats were randomly divided into five groups; groups 1, 2, 3, and 4 received 40%, 50%, or 60% acetic acid, or sodium hydroxide crystals, respectively, applied to the lower labial mucosa. All animals then received 50% acetic acid applied twice over the same area of the lower labial mucosa after the mucosa ulcer had healed. In the group 5, 50% acetic acid was applied to the buccal mucosa using a similar approach to examine the effects on formation of oral ulcers in different mucosal areas. The gross appearance of the mucosa, the ulcer formation rate, and the healing time were measured. Mucosal specimens were also taken to assess the histological features of the mucosal ulcer. Results Administration of 50% acetic acid to the lower labial mucosa produced the highest rate of ulcer formation, which showed a regular shape that allowed accurate measurement. The healing time and histological features of the ulcers in the groups 2 were similar to humans. By contrast, after the ulcers had healed, there was a low rate of new ulcers when applying 50% acetic acid over the lower labial gingival mucosa. Conclusions Oral ulcer induced by 50% acetic acid at the lower labial mucosa of Sprague Dawley rats is optimal for therapy evaluation. In addition, mucosa previously burnt by chemical regents are unsuitable for repeated establishment of an ulcer model.
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