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周琛云,朱晓霞,徐子林,周裕卿,唐琳,罗燕,阎锋.磨薄颅骨后大鼠经颅超声灌注成像表现及安全性评估[J].中国实验动物学报,2018,26(5):541~547.
磨薄颅骨后大鼠经颅超声灌注成像表现及安全性评估
Assessment of transcranial ultrasound perfusion and safety following skull thinning in rats
投稿时间:2018-05-09  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 05. 001
中文关键词:  超声灌注成像  经颅超声  大鼠  时间- 强度曲线
英文关键词:Ultrasound perfusion imaging  intracranial ultrasound  rat  time?intensity curve
基金项目:
作者单位E-mail
周琛云 四川大学华西医院超声科,成都 610041 echo_@163. com 
朱晓霞 1. 四川大学华西医院超声科,成都 610041
2. 四川大学华西医院超声影像药物研究室,成都 610041 
 
徐子林 四川大学华西临床医学院,成都 610041  
周裕卿 四川大学华西医院超声影像药物研究室,成都 610041  
唐琳 1. 四川大学华西医院超声科,成都 610041
2. 四川大学华西医院超声影像药物研究室,成都 610041 
 
罗燕 四川大学华西医院超声科,成都 610041  
阎锋 1. 四川大学华西医院超声科,成都 610041
2. 四川大学华西医院超声影像药物研究室,成都 610041 
yan_feng@ scu. edu. cn 
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中文摘要:
      目的 通过磨薄SD 大鼠局部颅骨改善声窗后观察右脑经颅超声灌注成像表现并行定量评估,初步评价磨骨及经颅超声对血?脑脊液屏障的影响?方法 将48 只SD 大鼠分为A?B 两组?A 组6 只大鼠在右侧局部颅骨磨薄前?后分别进行一次经颅超声灌注成像,比较成像效果?检查结束后,其中4 只大鼠行大脑依文思蓝 (EB)染色,2 只行头部核磁共振(MRI)检查,并均于处死后取两侧颅骨骨质做H&E 染色,测量磨头骨后剩余颅骨厚度?B 组42 只大鼠将右侧局部颅骨磨薄后行视交叉平面的右脑经颅超声灌注成像(团注0. 15 mL 超声造影剂SonoVueTM),并行动态超声造影分析(时间- 强度曲线)获取磨头骨后正常大鼠大脑经颅超声灌注成像参数?结果 SD 大鼠磨骨后右脑灌注成像效果改善明显,呈“快速增强?快速廓清,缓慢清除”的特点?时间- 强度曲线定量分析结果:峰值强度(PI)磨头骨后较磨头骨前约增加3 倍[(9. 98 ±2. 35)vs(3. 24 ±1. 49)dB, P < 0. 01],而达峰时间 (TTP)无明显改变[(4. 66 ±0. 45)vs(3. 86 ±1. 43)s, P > 0. 01]?右侧颅骨磨薄后的厚度约(66. 1 ±11. 4)μm;EB染色及MRI 检查未发现明显的血?脑脊液屏障破坏?结论 颅骨磨薄手术对SD 大鼠脑组织无明显损伤并可明显改善经颅超声灌注成像效果,该技术可用于小动物的经颅超声灌注成像的定量分析?这种微创?实时影像方法可用于各种小动物病理模型的脑血流动态变化的研究?
英文摘要:
      Objective To examine the efficacy and safety (blood?brain barrier permeability) of right hemisphere cerebral perfusion imaging using transcranial ultrasound following skull thinning in the rat. Methods Forty?eight Sprague Dawley rats received local skull?thinning surgery for transcranial ultrasound perfusion imaging (with injection of 0. 15 mL SonoVueTM ). Perfusion findings were compared before and after the skull surgery in six rats, and the brains were examined by magnetic resonance imaging (n = 2) or Evans blue staining (n = 4). The skulls of the rats were also removed for pathological examination to measure the thickness of the remaining skull. Perfusion in the right hemisphere of the brain was analyzed in 48 rats with a time?intensity curve to obtain quantitative transcranial ultrasound perfusion imaging parameters. Results After the surgery, the ultrasound perfusion imaging of the right hemisphere was improved, with typical perfusion patterns including a rapid wash?in and wash?out, and slow clearance of the contrast enhancement. The average peak intensity(PI)value after skull thinning was almost three times higher than that before surgery [(9. 98 ±2. 35) dB vs (3. 24±1. 49) dB, respectively; P < 0. 01], while the time to peak (TTP) was unchanged [(4. 66 ± 0. 45) s vs (3. 86 ±1. 43) s, respectively; P > 0. 01]. The thickness of the thinned skull after grinding was approximately (66.1 ± 11.4)μm. Evans blue staining and magnetic resonance imaging showed no observable blood brain barrier damagses. Conclusions Skull?thinning surgery is safe and can be applied to small animals for quantitative ultrasound imaging without a craniotomy. This minimally invasive and real?time imaging technique may be useful for dynamic studies of cerebral blood flow changes in various animal disease models.
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