Naked mole?rats ( NMR, Heterocephalus glaber) are a unique species of rodents exhibiting high longevity, tumor resistance, hypoxia tolerance and pain insensitivity. These features make NMRs widely used mammalian models for biomedical research in aging, cancer, neurobiology and other fields. Their laboratory animalization and application are very important. Thus, our research team carried out a systematic study on the establishment of laboratory animalization and standardization of NMRs. Through the study of reproduction, genetics, microbiology and nutrition of NMRs, the difficulties of low reproductive rate, unclear genetic background and unidentified pathogenic microorganisms have been overcome. The population of the ordinary closed NMRs was successfully established, and the present population has been propagated to the sixth generation. At the same time, the biological characteristics of the population have been systematically studied, such as hypoxia tolerance, tumor resistance and longevity, which will provide new strategies for the prevention and treatment for related diseases.