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戎亦骊,潘永明,黄俊杰,徐孝平,朱科燕,陈方明,陈民利.高脂高盐饮食诱导巴马小型猪高血压模型的建立及其机制探讨[J].中国实验动物学报,2018,26(4):474~479.
高脂高盐饮食诱导巴马小型猪高血压模型的建立及其机制探讨
Establishment of a Bama minipig model of hypertension induced by high fat and high salt diet and its mechanism
投稿时间:2018-04-02  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 04. 011
中文关键词:  高脂高盐饮食  高血压  小型猪  肾素- 血管紧张素系统?水通道蛋白2
英文关键词:high fat and high salt diet  hypertension  Bama minipigs  renin?angiotensin system  aquaporin?2
基金项目:浙江省科技厅公益性(No. 2017C37110)
作者单位E-mail
戎亦骊 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053 ryl@ zju. edu. cn 
潘永明 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
黄俊杰 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
徐孝平 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
朱科燕 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
陈方明 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053  
陈民利 浙江中医药大学动物实验研究中心/ 比较医学研究所,杭州 310053 cmli991@ zcmu. edu. cn 
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中文摘要:
      目的 采用高脂高盐饮食建立巴马小型猪高血压模型,并探讨其可能的发病机制?方法 取雄性巴马小型猪18 只,随机分成3 组:正常对照(NC)组?高脂(HF)组和高脂高盐(HFHS)组,每组6 只?NC 组饲喂普通饲料,HF 组和HFHS 组分别饲喂高脂饲料和高脂高盐饲料,连续24 周?在造模8 周?16 周和24 周时测量小型猪的收缩压(SBP)和舒张压(DBP),在造模24 周时测定血糖血脂和肝肾功能,以及血浆内皮素1(ET - 1)?肾素 (Renin)?血管紧张素II(Ang II)?水通道蛋白-2(AQP -2)?血管加压素(AVP)和血管内皮生长因子(VEGF)等指标,并取肝?肾进行组织病理学观察?结果 与NC 组比,HF 组和HFHS 组在造模8 周后SBP 和DBP 明显升高,并呈持续上升趋势,且HFHS 组高于HF 组;同时,造模24 周后HF 组和HFHS 组小型猪的体重和肝?肾指数均显著增加( P < 0. 05),且血浆TC?CREA 和ET -1 水平亦显著升高( P < 0. 05, P < 0. 01);而HFHS 组BUN 水平显著降低 ( P < 0. 05),但renin?Ang?II?AQP -2?AVP 含量均显著升高( P < 0. 05, P < 0. 01)?油红“O”染色结果显示,HF 组和HFHS 组肝?肾出现脂质沉积,且出现肾小动脉管壁增厚等病理改变?结论 高脂高盐饮食诱导8 周可建立小型猪高血压模型,其发病机制可能与影响肾脏功能进而激活RAS 系统和AVP?AQP -2 有关?
英文摘要:
      Objective To establish a Bama minipig model of hypertension induced by high?fat and high?salt diet and to explore its mechanism. Methods Eighteen healthy male Bama minipigs were randomly divided into 3 groups: normal control (NC) group, high?fat (HF) diet group and high?fat/ high?salt diet (HFHS) group, 6 in each group. The NC group was fed normal basal diet, the HF group and HFHS group were fed with high?fat diet and high?fat/ high?salt diet for 24 weeks, respectively. The systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured at 8, 16 and 24 weeks of modeling. The minipigs were weighed and the levels of blood glucose, lipids, liver and kidney function as well as the levels of endothelin - 1 (ET - 1), renin, angiotensin II (Ang?II), aquaporin - 2 (AQP - 2), vasopressin (AVP) and vascular endothelial growth factor (VEGF) were determined at 24 weeks after modeling. Meanwhile, samples of liver and kidney tissues were taken for histopathological examination. Results Compared with the NC group, SBP and DBP were significantly increased in the HF and HFHS groups after 8 weeks of modeling and showed a continuous rising trend, and the HFHS group was higher than that in the HF group. The body weight and liver and kidney coefficients were significantly increased in the HF and HFHS groups ( P < 0. 05), and levels of plasma TC, CREA and ET - 1 were significantly increased ( P < 0. 05, P < 0. 01). In addition, the level of BUN was significantly decreased ( P < 0. 05) and the levels of renin, Ang?II, AQP - 2 and AVP in the HFHS group were significantly increased ( P < 0. 05, P <0. 01). Oil red “O” staining showed lipid deposition in the liver and kidney tissues, and thickening of renal arterial wall and other pathological changes in the HF and HFHS groups. Conclusions A Bama minipig model of hypertension is successfully established by high fat and high salt diet for 8 weeks. Its pathogenesis may be related to the effect of alteration in renal function and activation of RAS system and AVP?AQP -2.
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