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黄俊杰,刘军平,陈民利,朱科燕,陈诚,吕涛,潘永明.自主神经功能在兔动脉粥样硬化中的作用及蜂王浆的干预[J].中国实验动物学报,2018,26(4):467~473.
自主神经功能在兔动脉粥样硬化中的作用及蜂王浆的干预
Role of autonomic nervous function in and the effect of royal jelly intervention on rabbit atherosclerosis
投稿时间:2018-03-21  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 04. 010
中文关键词:  自主神经功能  动脉粥样硬化  高脂高糖    蜂王浆
英文关键词:Autonomic nervous function  atherosclerosis  high fat and high sugar diet  rabbit  royal jelly
基金项目:浙江省科技厅公益性项目(No. 2016C37141)
作者单位E-mail
黄俊杰 浙江中医药大学动物实验研究中心,杭州 310053  
刘军平 浙江中医药大学动物实验研究中心,杭州 310053 liujp89day@126. com 
陈民利 浙江中医药大学动物实验研究中心,杭州 310053  
朱科燕 浙江中医药大学动物实验研究中心,杭州 310053  
陈诚 浙江中医药大学动物实验研究中心,杭州 310053  
吕涛 浙江中医药大学动物实验研究中心,杭州 310053  
潘永明 浙江中医药大学动物实验研究中心,杭州 310053 pym918@126. com 
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中文摘要:
      目的 探讨自主神经在兔动脉粥样硬化(AS)中的作用及蜂王浆干预效果?方法 取雄性日本大耳白兔18 只,随机分为正常对照(NC)组?高脂高糖饮食(HFHSD)组?蜂王浆治疗(RJ)组,每组6 只?采用高脂高糖饮食12 周诱发AS 模型,同时RJ 组每次口服200 mg/ kg 蜂王浆,2 次/ 日?造模结束后,取血测定血脂[总胆固醇 (TC)?三酰甘油(TG)?低密度脂蛋白胆固醇(LDL?C)和高密度脂蛋白胆固醇(HDL?C)]?超氧化物歧化酶(SOD)?丙二醛(MDA)和一氧化氮(NO)水平;并测量心电图和血压,分别测量心率变异性(HRV)和心血管压力反射敏感性(BRS)反应;取血管进行HE 病理观察和苏丹IV 染色评估AS 程度?结果 与NC 组比,HFHSD 组血脂升高( P < 0. 05),主动脉有明显的脂质沉积和AS 斑块( P < 0. 05, P < 0. 01),血浆SOD 活性和NO 水平下降且MDA 含量升高( P < 0. 05, P < 0. 01),心功能降低的同时伴有HRV 和BRS 值降低( P < 0. 05, P < 0. 01);与HFHSD 组比,蜂王浆治疗组能明显改善上述指标?结论 高脂高糖饮食能导致兔心脏自主神经功能紊乱,并促进AS 发生,蜂王浆干预后能调整自主神经功能,延缓AS 病变?
英文摘要:
      Objective To investigate the role of autonomic nervous function and the effect of royal jelly intervention on rabbit models of atherosclerosis (AS). Methods Eighteen healthy 3 - 4?month old male Japanese white rabbits were randomly divided into normal control (NC) group, high?fat high?sugar diet (HFHSD) group and royal jelly intervention (RJ) group, 6 rabbits in each group. The rabbits received high?fat and high?sugar diet for 12 weeks to induce atherosclerosis model. At the same time, the RJ group was orally given 200 mg/ kg royal jelly daily, twice a day, for consecutive 12 weeks. The plasma levels of total cholesterol (TC), triglyceride (TG), low?density lipoprotein cholesterol (LDL?C) and high?density lipoprotein cholesterol (HDL?C), superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) were detected. The electrocardiogram and blood pressure were monitored, and the heart rate variability (HRV) and baroreflex sensitivity (BRS) were analyzed. The pathological changes in the abdominal aorta tissues were observed using H&E and Sudan IV staining, respectively. Results Compared with the NC group, the levels of serum lipids in the HFHSD group were significantly increased ( P < 0. 05). The lipid deposition and atherosclerotic plaque sizes in the abdominal aorta were significantly increased ( P < 0. 05). The plasma SOD and NO levels were decreased and the MDA content was increased ( P < 0. 05, P < 0. 01). The reduction of cardiac function was accompanied by a decrease of HRV and BRS values ( P < 0. 05, P < 0. 01). Compared with the HFHSD group, RJ significantly improved the above indicators. Conclusions High fat and high sugar diet can lead to cardiac autonomic nervous dysfunction and to further form atherosclerosis in rabbits. Royal jelly intervention can regulate cardiac autonomic nervous function and delay the development of atherosclerotic lesions.
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