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侯改霞,肖国强,习雪峰,刘倩倩.糖尿病大鼠心肌纤维化与心肌AGEs/ RAGE 水平的变化[J].中国实验动物学报,2018,26(4):461~466.
糖尿病大鼠心肌纤维化与心肌AGEs/ RAGE 水平的变化
Changes of myocardial fibrosis and AGEs / RAGE levels in diabetic rats
投稿时间:2018-02-20  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 04. 009
中文关键词:  高脂饮食  链脲佐菌素  Ⅱ型糖尿病  心肌纤维化  AGEs/ RAGE  SD 大鼠
英文关键词:high?fat fodder  streptozotocin  type II diabetes  myocardial fibrosis  AGEs/ RAGE  S?D rats
基金项目:河南省教育厅科学技术研究重点项目(No. 14B890010)
作者单位E-mail
侯改霞 河南大学体育学院,河南开封 475001 hgx715@163. com 
肖国强 华南师范大学体育科学学院,广州 510006  
习雪峰 河南大学体育学院,河南开封 475001  
刘倩倩 河南大学体育学院,河南开封 475001  
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中文摘要:
      目的 通过构建Ⅱ型糖尿病大鼠模型,研究该模型心肌纤维化和心肌AGEs/ RAGE 水平的变化情况?方法 雄性SD 大鼠高脂饲料饲喂6 周后,过夜空腹12 h,腹腔注射小剂量链脲佐菌素(STZ,30 mg/ kg)?注射3?7 d 后检测大鼠随机血糖浓度,两次随机血糖≥16. 7 mmol/ L 为Ⅱ型糖尿病大鼠建模成功?在建模成功后第4?8和12 周,检测大鼠血FBG?insulin 和左心室AGEs?Hyp 含量及Masson 染色?RAGE 表达的变化?结果 造模成功后第4?8 和12 周,糖尿病对照组(4D,8D,12D)血FBG 水平均较同周次正常对照组(4C,8C,12C)显著升高,体重显著下降;造模成功后第12 周时,糖尿病对照组(12D)大鼠Insulin 显著高于同周次正常对照组(12C)和4 周时糖尿病对照组(4D);从造模成功后第8 周开始,糖尿病对照组(8D,12D)心肌Hyp?CVF?AGEs 和RAGE 与正常对照组 (8C,12C)相比均显著升高;2 型糖尿病大鼠心肌AGEs/ RAGE 水平与CVF 呈显著正相关?结论 高脂饮食饲喂加腹腔注射小剂量STZ,能够成功复制Ⅱ型糖尿病大鼠模型?随着病程的延长,心肌纤维化和心肌AGEs 含量?RAGE蛋白表达均呈逐渐上升趋势,且Ⅱ型糖尿病大鼠心肌AGEs/ RAGE 水平与心肌纤维化存在有正相关关系?
英文摘要:
      Objective To established a rat model for type II diabetes and to investigate the gradual changes of myocardial fibrosis and myocardial AGEs/ RAGE levels in type II diabetes. Methods Male SD rats were fed high?fat fodder for 6 weeks, and then injected a small dose of streptozotocin (STZ, 30 mg/ kg) after overnight fasting for 12 h. The random blood GLU was detected on the 3th and 7th days, respectively, after STZ injection. The rats with random blood GLU concentration higher or equal to 16. 7 mmol/ L detected twice were considered as successful rat model of type II diabetes. Six rats of group C and six rats of group D fasting for 12 h were killed after intraperitoneal anesthesia at the 4th, 8th and 12th weeks after the successful establishment of type II diabetes models. Blood samples were taken for FBG and insulin detection. A portion of the left ventricle tissue was used for Masson staining and immunohistochemical detection of RAGE, and the other portion of the left ventricle was used for the detection of content of AGEs and Hyp. Results The FBG levels of group D were significantly higher, and the body weights of group D were significantly lower than group C at the same weeks. Blood insulin of the group D at the 12th week was significantly higher than that of the group C at the 12th week and group D at the 4th week. Myocardial Hyp, CVF, AGEs and RAGE of group D were significantly higher than those of the group C at the 8th, 12th weeks. The AGEs/ RAGE level of myocardium in the diabetic rats was positively correlated with CVF. Conclusions The model of type II diabetes is successfully established through intraperitoneal injecting a low?dose streptozotocin to SD rats which had been fed high?fat fodder. The rat models of type II diabetes demonstrate myocardial fibrosis at the 12th week after the successful establishment of diabetes. With the further development of diabetes, the myocardial fibrosis, content of myocardial AGEs, RAGE protein expression of the diabetic rats show an upward trend. The level of AGEs/ RAGE is positively correlated with the degree of myocardial fibrosis of type II diabetic rats.
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