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纪润璧,马吉春,闻向梅,林江.大鼠胃炎癌转化模型建立与早期诊断方法探索[J].中国实验动物学报,2018,26(4):444~447.
大鼠胃炎癌转化模型建立与早期诊断方法探索
Construction of gastritis transformation into cancer model of rat and preliminary exploration of early diagnosis of gastric cancer
投稿时间:2018-04-23  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 04. 006
中文关键词:  动物模型  胃癌  炎癌转化  早期诊断
英文关键词:animal model  gastric cancer  transformation from gastritis to cancer  early diagnosis
基金项目:国家自然科学基金青年项目(No. 81702429);江苏省自然科学基金青年项目(No. BK20170561);镇江市社会发展项目(No. SH2016047)
作者单位E-mail
纪润璧 江苏大学附属人民医院,江苏镇江 212002 runbiji@163. com 
马吉春 江苏大学附属人民医院,江苏镇江 212002  
闻向梅 江苏大学附属人民医院,江苏镇江 212002  
林江 江苏大学附属人民医院,江苏镇江 212002 2651329493@ qq. com 
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中文摘要:
      目的 构建大鼠胃炎癌转化模型,探索新的胃癌早期血清学诊断指标?方法 采用N - 甲基?N′ -硝基?N′ - 亚硝基胍饮水(MNNG)协同高盐饲料喂养的方法诱导大鼠胃炎癌转化模型?核磁共振成像及组织病理学观察大鼠胃部恶化过程?qRT?PCR 检测不同时间点(造模第0,12,24,36,48 周)大鼠血清miR - 17 - 5p 和miR-374 -5p 表达水平?结果 MNNG 饮水协同高盐饲料喂养成功诱导大鼠胃炎癌转化,经历萎缩性胃炎(造模第24 周)和不典型增生(造模第48 周)的过程?血清中miR -17 -5p 和miR - 374 - 5p 的表达水平与大鼠胃部恶化程度正相关?与正常对照组相比,第36?48 周时,miR -17 -5p 表达水平分别增加约8 倍(t = 4. 12, P < 0. 001)和10 倍(t =5. 33, P < 0. 001);第48 周时,miR -374 -5p 表达水平增加约6 倍(t =3. 62,0. 001 < P < 0. 002)?结论MNNG 饮水协同高盐饲料喂养构建的大鼠胃炎癌转化模型为研究胃癌发病机理提供了良好的动物模型,血清miR-17 -5p 和miR -374 -5p 是潜在的胃癌早期创诊断指标?
英文摘要:
      Objective To construct a rat model of transformation from gastritis to cancer and to explore possible serological index for early diagnosis of gastric cancer. Methods N?methyl?N′?nitro?N′?nitrosoguanidine (MNNG) in drinking water combined with high salt feed were used to induce gastric carcinogenesis in rats. Dynamic observation of gastric changes in the rats was carried out by nuclear magnetic resonance imaging and histopathology. The expression of serum miR -17 -5p and miR -374 -5p of rats were detected by qRT?PCR at different time points (0th, 12th, 24th, 36th, 48th weeks). Results MNNG combined with high salt diet induced gastric alterations, through the atrophic gastritis (the 24th week) and atypical hyperplasia (the 48th week) process. The expressions of serum miR -17 -5p and miR -374 -5p were positively correlated with the degree of gastric changes in the rats. Compared with the normal control group, the expression of miR -17 -5p was increased about 8 times (t =4. 12, P < 0. 001) and 10 times (t =5. 33, P < 0. 001) at the 36th and 48th week, respectively, and the expression of miR -374 -5p increased by about 6 times (t =3. 62, 0. 001 < P < 0. 002) at the 48th week. Conclusions The rat model of transformation from gastritis into cancer is constructed by MNNG drinking water combined with high salt feed. It provides a good animal model for studying the pathogenesis of gastric cancer. Our findings preliminarily indicate that serum MiR -17 -5p and miR -374 -5p may be potential markers for non?invasive diagnosis of gastric cancer at the early stage.
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