首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
曹敏,向钦,刘阳,何明忠,赖国旗,衣启营.CBA/ CaJ 小鼠脏器重量?血液生理生化指标及免疫细胞的比例[J].中国实验动物学报,2018,26(4):418~423.
CBA/ CaJ 小鼠脏器重量?血液生理生化指标及免疫细胞的比例
Organ weights, blood physiological and biochemical indices and proportion of immune cells in CBA / CaJ mice
投稿时间:2018-02-07  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 04. 002
中文关键词:  CBA/ CaJ 小鼠  脏器重量  血液生理生化指标  血液生化指标  免疫细胞
英文关键词:CBA/ CaJ mouse  organ weight  physiological indices  blood  biochemical indices  Immune cells
基金项目:重庆市科委社会民生项目(No. cstc2017shmsA130097);国家自然科学基金资助项目(No. 81570541);重庆市教委科研项目 (No. KJ1702013)
作者单位E-mail
曹敏 重庆医科大学实验动物中心,重庆 400016 1126870237@ qq. com 
向钦 重庆医科大学实验动物中心,重庆 400016  
刘阳 重庆医科大学实验动物中心,重庆 400016  
何明忠 重庆医科大学实验动物中心,重庆 400016  
赖国旗 重庆医科大学实验动物中心,重庆 400016  
衣启营 重庆医科大学实验动物中心,重庆 400016 109180604@ qq. com 
摘要点击次数: 80
全文下载次数: 18
中文摘要:
      目的 测定SPF 级CBA/ CaJ 小鼠的基础生物学指标,为更好利用该品系小鼠,从事生物医药等研究工作奠定基础?方法 选取6 周龄和12 周龄的CBA/ CaJ 雌性和雄性小鼠,分别称量不同性别小鼠的主要脏器重量,应用全自动血液生理?生化分析仪检测血液生理?生化指标并利用流式细胞仪检测外周血免疫细胞比例?结果 主要脏器重量显示同周龄(6 周或12 周)雌雄小鼠相比较,雄鼠体重?心脏?肝?肾重量均显著高于雌鼠( P < 0. 01);同性别CBA/ CaJ 小鼠,12 周龄雄鼠体重显著高于6 周龄( P =0. 000)?血液学生理指标显示6 周龄雄鼠MCV?RDW?PLT?P?LCR?BUN 及CHOL 均显著高于雌鼠( P < 0. 01),而雄性的MCHC 显著低于雌鼠( P =0. 000)?血液学生化指标显示12 周龄雄鼠的ALKP 显著低于雌鼠( P = 0. 001),CHOL 显著高于雌鼠( P = 0. 000)?12 周龄同性别CBA/ CaJ 小鼠MCV?RDW?MCH 及ALKP 明显低于6 周龄( P < 0. 01),而BUN 显著高于6 周龄( P < 0. 01)?外周血免疫细胞比例显示该品系小鼠B 细胞?T 细胞?NK 细胞和粒细胞都占有一定比例?结论 建立了CBA/ CaJ 小鼠的基础生物学数据?CBA/ CaJ 小鼠是具有免疫活性的小鼠,其龄期和性别因素对其基础生物学指标都有一定的影响?
英文摘要:
      Objective To make better use of CBA/ CaJ mice in biomedical research, the basic biological indicators of SPF CBA/ CaJ mice were determined. Methods CBA/ CaJ mice at six and twelve weeks of age were selected, and the main organ weights were detected. The blood physiological and biochemical parameters, and proportion of immune cells were measured using a full?automatic blood physiological and biochemical analyzer and flow cytometer, respectively. Results Compared with the male and female mice at the same age (6 or 12 weeks), body weight, heart, liver, kidney weight of males were significantly higher than females ( P < 0. 01). Mice with the same genders, the body weight of male mice at 12 weeks was significantly higher than the 6?week old male mice ( P =0. 000). Compared the male and female mice at six weeks, blood physiological parameters showed that MCV, RDW, PLT, P?LCR, BUN and CHOL of males were significantly higher than females ( P < 0. 01), while MCHC of male was significantly lower than female ( P =0. 000). Blood biochemical indexes showed that ALKP of male mice was lower than female at 12 weeks( P =0. 001), while CHOL was higher than female ( P = 0. 000). Mice with the same genders and different weeks, MCV, RDW, MCH and ALKP of 12?week old mice were significantly lower than six weeks ( P < 0. 01), while BUN was higher than six weeks mice ( P < 0. 01). The result of blood immune cell analysis showed that all mice had a certain proportion of B, T, NK cells and granulocytes. Conclusions The basic biological data of CBA/ CaJ mice are established. The result suggest that the cellular immune system for the CBA/ CaJ mice is intact, and these indicators are affected by age and gender.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 840569 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb1@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码