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莫伟彬,杨衍滔,郭艳菊,周彩华,邵晓云.益生菌干预对运动大鼠胃肠激素与AQP4 表达的影响[J].中国实验动物学报,2018,26(4):411~417.
益生菌干预对运动大鼠胃肠激素与AQP4 表达的影响
Effect of probiotics on gastrointestinal hormones and expression of AQP4 in rats during exercise
投稿时间:2018-03-27  
DOI:10.3969/j. issn. 1005 - 4847. 2018. 04. 001
中文关键词:  益生菌  力竭运动  胃肠激素  水通道蛋白-4  大鼠
英文关键词:probiotics  exhaustive exercise  gastrointestinal hormones  aquaporin 4  rats
基金项目:国家自然科学基金资助项目(No. 21562006,No. 31060121);教育发展基金会“教师成长基金”项目(No. EDF2016005);广西教育厅科研项目(No. 2016JGY73);国家重点实验室开放项目(No. CMEMR2012?B04)
作者单位E-mail
莫伟彬 1. 广西师范大学体育学院,广西桂林 541004
2. 药用资源化学与药物分子工程国家重点实验室广西桂林 541004 
mogaol@163. com 
杨衍滔 广西师范大学体育学院,广西桂林 541004  
郭艳菊 广西师范大学体育学院,广西桂林 541004  
周彩华 广西师范大学体育学院,广西桂林 541004  
邵晓云 桂林医学院人体解剖学教研室,广西桂林 541004 576941624@ qq. Com 
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中文摘要:
      目的 研究益生菌干预下力竭性运动大鼠胃肠道功能的影响?方法 40 只SD 雄性大鼠随机分为安静对照组(NC),运动对照组(ME),安静给药组(NCY),运动给药组(MEY)?ME 与MEY 组进行6 周力竭训练,速度为19. 3 m/ min,坡度为5o,每天训练1 次,每周6 d?NCY 与MEY 组每日灌胃浓度为107 CFU/ mL 益生菌溶液10. 0 mL/ (kg·d)?6 周力竭训练结束后,检测大鼠血清中胃肠激素ghrelin?PYY?CCK?GLP -1?MTL 和GAS 指标含量,并采用RT?PCR 和Western blot 法检测大鼠胃和结肠组织中AQP4 的表达?结果 ME 组大鼠血清ghrelin 和PYY 含量均高于NC 组( P < 0. 01),而ME 组CCK?GLP -1?MTL 和GAS 含量均低于NC 组( P < 0. 01);NCY 组大鼠血清ghrelin?PYY 均低于ME 组( P < 0. 01),而CCK?GLP -1?MTL 和GAS 含量均高于ME 组( P < 0. 01);MEY组大鼠血清ghrelin 高于NC 组( P < 0. 05)而低于ME 组( P < 0. 01);MEY 组大鼠血清PYY 的含量低于ME 组( P < 0. 01);MEY 组大鼠血清CCK 含量低于NC 组( P < 0. 05)而高于ME 组( P < 0. 05);MEY 组大鼠血清GLP -1?MTL 和GAS 含量均高于ME 组( P < 0. 01)?ME 组大鼠胃肠组织AQP4 mRNA 和蛋白表达均低于NC 组( P <0. 01 或 P < 0. 05);NCY 和MEY 组大鼠胃肠组织AQP4 mRNA 和蛋白表达均高于ME 组( P < 0. 01 或 P <0. 05)?结论 力竭性运动引起大鼠胃肠激素含量发生变化以及AQP4 表达的上升与补充益生菌溶液密切相关,提示益生菌可能参与了胃肠激素的分泌和水代谢的过程?
英文摘要:
      Objective To study the effect of intervention of probiotics on gastrointestinal function and expression of AQP4 in rats during exhaustive exercise. Methods Forty male SD rats were randomly divided into quiet control group (NC), exercise control group (ME), quiet medication group (NCY) and exercise medication group (MEY). The exercise control group and the exercise medication group were subjected to exhaustive training for 6 weeks: the running speed was 19. 3 m/ min, the slope was 5°, the training was once a day, 6 d/ week for 6 weeks. Probiotics in concentration of 107 CFU/ mL was gavaged 10. 0 mL/ (kg/ d) in the quiet medication group and exercise medication group. After 6 weeks of exhaustive training, the activities of serum gastrointestinal hormones ghrelin, PYY, CCK, GLP -1, MTL and GAS were measured. . The expression of AQP4 was detected by RT?PCR and western blot. Results The contents of serum ghrelin and PYY were significantly higher in the ME group than those in the NC group ( P < 0. 01). The contents of intestinal CCK, GLP -1, MTL and GAS in the ME group were significantly lower than those in the NC group ( P < 0. 01). The serum ghrelin and PYY were lower in the NCY group than in the ME group ( P < 0. 01), while the contents of intestinal CCK, GLP -1, MTL and GAS in the NCY group were higher than those in the ME group ( P < 0. 01). The serum ghrelin in the MEY group was higher than that in the NC group ( P < 0. 05) and lower than that in the ME group ( P < 0. 01). The PYY in the MEY group was lower than that in the ME group ( P < 0. 05). The serum CCK was lower in the MEYgroup than that in the NC group ( P < 0. 05) and higher than that in the ME group ( P < 0. 05). The serum GLP -1, MTL and GAS in the MEY group were higher than those in the ME group ( P < 0. 01). The expression of AQP4 mRNA and protein in the gastrointestinal tissue of the ME group were lower than those in the NC group ( P < 0. 01 or P < 0. 05). The expression of AQP4 mRNA and protein in the NCY and MEY groups were higher than those in the ME group ( P < 0. 01 or P < 0. 05). Conclusions Exhaustive exercise causes changes in gastrointestinal hormone levels and increase of AQP4 expression in rats. Probiotics may be involved in the gastrointestinal hormone secretion and water metabolic process.
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