At present, the cats play a more and more important role in medical experiments as an experimental animal, especially for the studies of neurology, physiology and toxicology. Compared with rodent animals, the physiological characteristics, anatomical features, pathological and biochemical reactions of cats are closer to human beings, and compared with the primate animals, they have advantages of economy, abundant resources and so on. Therefore, cat has an extensive application prospect in animal models of human diseases. This article mainly reviews and summarizes the establishing method and research status of cats as an animal model of human diseases in ophthalmology, nervous system, tumor and other fields in recent years.