首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
武亚琦,钟根深,吴敏娜.小鼠粪便及肠道各部位内容物细菌群落结构差异分析[J].中国实验动物学报,2015,23(3):249~255.
小鼠粪便及肠道各部位内容物细菌群落结构差异分析
Differential analysis of the bacterial community composition in mouse feces and intestinal contents
投稿时间:2015-01-04  
DOI:10.3969/j.issn.1005-4847.2015.03.006
中文关键词:  末端限制性片段长度多态性  变性梯度凝胶电泳  荧光定量PCR  小鼠  肠道微生态
英文关键词:Terminal restriction fragment length polymorphism (T-RFLP)  Degeneration gradient gel electrophoresis (DGGE)  Real-time fluorescent quantitative PCR (qPCR)  Mouse  Intestinal microbiota
基金项目:国家自然科学基金(40901125,81201765);新乡医学院科研培育基金(2013ZD102)。
作者单位E-mail
武亚琦 新乡医学院基础医学院, 河南 新乡 453003  
钟根深 新乡医学院第一附属医院, 河南 卫辉 453100  
吴敏娜 新乡医学院基础医学院, 河南 新乡 453003 minnawu@xxmu.edu.cn 
摘要点击次数: 1111
全文下载次数: 1246
中文摘要:
      目的 分析小鼠粪便及肠道各部位内容物细菌群落结构差异,探讨粪便取样研究肠道微生物与疾病关系的科学性和客观性,为相关实验设计提供参考。 方法 利用末端限制性片段长度多态性(T-RFLP)、变性梯度凝胶电泳(DGGE)和荧光定量PCR(qPCR)技术对BALB/c小鼠肠道不同部位(十二指肠、空肠、回肠、盲肠、结肠和直肠)内容物及粪便中细菌群落组成和丰度进行比较,分析细菌群落在小鼠粪便及肠道各部位的分布差异。 结果 粪便与直肠、结肠中优势片段均为244、255 bp和449 bp,而小肠内容物(十二指肠、空肠及回肠)的优势片段则为60、73、261、268 bp和272 bp,且小肠各部位之间细菌群落结构差异较大;十二指肠和空肠内容物中的细菌丰度较低,分别为6.9 log (copies)/g和8.3 log (copies)/g,而粪便中细菌丰度高达11.8 log (copies)/g,约2倍于十二指肠细菌丰度,显著高于十二指肠和空肠(P < 0.05),与大肠各部位及回肠内容物细菌丰度相当,差异不显著(P > 0.05)。结论 大肠各部位内容物和粪便的细菌群落结构相似,个体差异较小肠小,适合通过粪便取样进行大肠部位特别是结直肠的肠道微生态与某些疾病的相关分析。
英文摘要:
      Objective To explore the objectivity and scientificity of fecal sampling, and to provide reference for investigating the relationship between intestinal microbes and diseases. Methods Terminal restriction fragment length polymorphism, degeneration gradient gel electrophoresis and real time fluorescent quantitative PCR techniques were applied to differentially analyze the bacterial community composition and abundance of intestinal contents and feces taken from different sites of BALB/c mouse intestine. Results The predominant T-RFLP fragments (T-RFs) in feces in the rectum and colon were 244 bp, 255 bp and 449 bp, however, those in feces of the small intestine including duodenum, jejunum and ileum were 60 bp, 73 bp, 261 bp, 268 bp and 272 bp, and with a larger variation of the bacterial community composition in various parts of the small intestine. The bacterial abundance in the contents of duodenum and jejunum were 6.9 log (copies)/g and 8.3 log (copies)/g, fewer than in the other parts of the intestine, while the bacterial abundance in the feces was as high as 11.8 log (copies)/g, being about 2 times higher than that in the duodenum and jejunum (P<0.05), and similar to that in the ileum and colon content (P>0.05). Conclusions The inter-mouse variations of bacterial community composition in the large intestine contents are small. The bacterial composition and abundance are similar suggest that studies on the relationship between large intestine especially colorectal microbiota and diseases may be conducted via fecal sampling.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 1302329 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb1@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码