Objective To monitor the growth and reproductive performance of a rare closed colony of Gobiocypris rarus (Ihb: IHB) and to provide basic information for its management and application. Methods Egg production, spawning interval, diameter of egg membrane, fertilization rate and hatching rate were monitored for 50 pairs of parents fish of wild gathered fish (P0), F1, F2, F3 and F4 generations. Moreover, body size of newly hatched offspring (F1 to F4), and at 7, 30, 60 and 90 days were measured to examine their growth profile. Results There were certain differences in growth between generations. F1 had a larger total length of the newly hatched larva and 7-day old larva than those of F2 to F4. But while in the fish at 30, 60 and 90 days, those of F2, F3, and F4 had a larger body size than that of F1, and exhibited higher growth indexes. Good performance on fertilization rate and hatching rate was shown, though there was certain fluctuation between generations. No difference of spawning interval of the closed colony were found between generations. The wild gathered fish (P0) seemed to have larger egg diameter and egg production per batch than those of F1 to F4. Conclusions It is suggested that differences in growth of the fish may be related with culture conditions, and the higher egg production may be related with the larger body size of P0. The results indicate that the closed colony preserves good performance of growth and reproduction. No increasing or decreasing tendency is found during the passage process. This Ihb: IHB colony meets the requirement of quality control of laboratory animal closed colony.