小鼠心肌梗死模型的建立及系统性评价
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1. 西安交通大学医学部转化研究院心血管研究所,西安 710061;2. 西安交通大学第一附属医院心血管内科,西安 710061;3. 西安交通大学医学部基础医学院实验动物学系,西安 710061

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Establishment of a mouse myocardial infarction model and systematic evaluation
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1.Institute of Cardiovascular Science, Translational Medicine Institute, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China. 2. Department of Cardiology, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061. 3. Department of Laboratory Animal Science, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an 710061

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    摘要:

    目的 构建小鼠心肌梗死模型,并进行系统性评价。 方法 42 只 C57BL/ 6J 小鼠分为两组:假手术组(n= 8)和模型组(n= 34)。 采用挤心脏法结合冠状动脉左前降支(LCA)结扎建立小鼠心梗模型。 术后 24 h、3 d、7d 分别进行心动超声评价心功能,打开胸腔观察梗死情况;术后 24 h 留取心脏标本进行 TTC 染色明确梗死发生;术后3 d 检测梗死区炎症标志基因表达情况;术后 7 d 进行 HE 染色并检测梗死区纤维化相关基因表达情况。 结果 与假手术组比,模型组小鼠可明确观察到左室前壁发白,TTC 染色明确梗死区域,提示模型建立成功。术后心超可见模型组心功能显著下降,且随时间延长逐渐加重。 术后 3 d 梗死区促炎基因 TNF-α 和 MCP-1 表达显著增加(P< 0. 05)。 术后 7 d HE 染色见梗死区大量炎性细胞浸润,梗死区与非梗死区界线分明;梗死区纤维化基因 FN 和 COL3A1 表达显著增加(P < 0. 01)。 结论 成功建立小鼠心梗模型及其基本评价体系,可作为模型建立的标志性指标。

    Abstract:

    Objective To establish a myocardial infarction mouse model for systematic evaluation. Methods A total of 42 C57BL/ 6J mice were divided into two groups: sham group ( n= 8) and model group ( n= 34). Myocardial infarction was induced by squeezing the heart and left anterior descending coronary artery ligation. Echocardiography was performed to evaluate cardiac functions at 24 h, 3 d and 7 d after surgery. Then, the chest was opened and the occurrence of myocardial infarction was evaluated. Heart samples were collected for TTC staining to confirm the occurrence of infarction at 24 h after surgery. Heart sections were prepared for HE staining at 7 d after surgery. mRNA expression of inflammatory and fibrotic related genes in the infarct area was detected at 3 and 7 d after surgery, respectively. Results After surgery, the left ventricular anterior wall of the model group showed a whitish myocardium compared with the sham group. TTC staining showed a white infarct area, suggesting that the model was established successfully. Echocardiography indicated that cardiac functions of the model group were significantly decreased and gradually deteriorated over time. Expression of inflammatory genes, such as TNF-α and MCP-1, was increased significantly at 3 days postoperatively in the infarct area (P< 0. 05). Histological analysis showed that many inflammatory cells had infiltrated into the infarct area at 7 days postoperatively, and the boundary between the infarct area and non-infarct area was clear. Expression of fibrotic genes, including FN and COL3A1, in the infarct area were increased significantly ( P< 0. 01). Conclusions The MI mouse model and its evaluation were successfully established and refined, which provide insights for model establishment.

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丁怡铭,高杰,邢元铭,温尔刚,夏聪聪,赵四海,刘恩岐,袁祖贻,白亮.小鼠心肌梗死模型的建立及系统性评价[J].中国实验动物学报,2022,30(7):873~879.

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  • 收稿日期:2022-05-12
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  • 在线发布日期: 2023-03-10
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