Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China
目的 通过慢性温和不可预知刺激( chronic unpredictable and mild stress,CUMS)建立 SD 大鼠抑郁模型,初步探讨不同风格音乐改善 SD 大鼠抑郁样行为的效果和机制。 方法 通过孤养后持续 4 周慢性不可预知刺激来构建 CUMS 大鼠,以糖水偏好实验(sucrose preference test,SPT)和强迫游泳实验( forced swimming test,FST) 筛选出 39 只 CUMS 抑郁大鼠,随机均分为模型组、轻音乐组、古典音乐组,并另设一组不做任何刺激的对照组作空白对照。 轻音乐组,古典音乐组各自每天播放久石让音乐、莫扎特音乐 4 h,持续 3 周后立即进行 SPT,FST 和 O 迷宫实验(O-maze)测试。 测试结束后立即取材。 HE 染色观察海马组织的结构变化、免疫组化和 Western Blot 检测脑源性神经营养因子(brain derived neurotrophic factor,BDNF)的表达水平、液相色谱串联质谱( liquid chromatography- tandem mass spectrometry, LC-MS / MS ) 检测海马组织中皮质酮 ( corticosterone, CORT )、 五羟色胺 ( 5- hydroxytryptamine,5-HT)含量。 ELISA 法检测外周血 BDNF、CORT、5-HT 和 IL-1β 的浓度变化。 结果 与模型组相比,轻音乐组与古典音乐组大鼠的糖水偏好百分比均上升(P< 0. 05)、FST 不动时间均缩短(P< 0. 01)、进入 O 迷宫开放臂的次数与停留时间均增加(P< 0. 05)。 与模型组相比,轻音乐组和古典音乐组的海马组织结构完整、排列整齐,BDNF 表达水平均升高(P< 0. 01)。 两组音乐干预后,血清 BDNF 水平均显著升高(P< 0. 01)。 两音乐组海马中 CORT 含量均降低(P< 0. 01),古典音乐组血清 CORT 含量在古典音乐组显著降低(P< 0. 01)。 血清 5-HT 含量在轻音乐组升高(P< 0. 01),但在古典音乐组无显著性差异。 两音乐组血清 IL-1β 浓度均显著降低(P< 0. 01)。 结论 古典音乐和轻音乐均可以在一定程度上改善抑郁模型大鼠的行为。
Objective To explore the effects and mechanism of different styles of music on the depression-like behavior of rats induced by chronic unpredictable and mild stress (CUMS). Methods We selected 39 depressed rats by sucrose preference tests (SPT) and forced swimming tests (FST). They were randomly divided into Control (no music), Light music, and Classical music groups. The Light music and Classical music groups were introduced to music by Hisaishi and Mozart, respectively, for 4 hours per day for 3 weeks, after which SPT, FST, and O-maze tests were conducted. A pathological change in the hippocampus was observed with HE staining. The expression level of hippocampal BDNF protein was detected by Western blot and immunohistochemical staining. Liquid chromatography-tandem mass spectrometry (LC- MS / MS) was used to measure the content of hippocampal CORT and 5-HT. BDNF, CORT, 5-HT, and IL-1 (interleukin- 1β, IL-1β) levels in serum were determined by ELISA. Results Compared with the Control group, the percentage of sucrose preference was significantly greater (P< 0. 05), the immobile time in FST was significantly less (P< 0. 01), and the time and staying time in the open arms of the O-maze were significantly greater (P< 0. 05) in the Light and Classical music groups. The hippocampal tissue of rats in the Light and Classical music group was relatively intact. The BDNF expression level in the hippocampus (P< 0. 01) and serum was significantly greater (P< 0.01) in the music groups. The CORT content in the hippocampus was significantly less in the music groups (P< 0. 01) and in the serum of only the Classical music group. The 5-HT content in the serum of the Light music group was greater (P< 0. 01) than the other groups. The content of IL-1β in the serum in both music groups was less than the Control group (P< 0. 01). Conclusions Light and classical music improve the behavior of depressed rats, indicating the potential value of music therapy in mental disease treatments.
李懿,曾莉,何丽雯,谭冬梅,张倩,谭毅.不同风格音乐对 CUMS 抑郁大鼠行为的影响及机制研究[J].中国实验动物学报,2022,30(3):384~391.复制