1. 北京中医药大学东方医院,北京 100078; 2. 北京中医药大学中医养生学北京市重点实验室,北京 100029; 3. 陕西中医药大学第一临床医学院,西安 711301
1.DongFang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China. 2. Key Laboratory of Health Cultivation of Beijing, Beijing University of Chinese Medicine, Beijing 100029. 3. the First School of Clinical Medicine, Shaanxi University of Chinese Medicine, Xi’an 711301
目的 检测甲炎康泰颗粒剂对自身免疫性甲状腺炎大鼠免疫相关细胞因子的影响,探讨甲炎康泰颗粒剂改善自身免疫性甲状腺炎的作用机制。 方法 30 只雌性 Lewis 大鼠随机选取正常组 10 只,其余 20 只采用皮下注射甲状腺球蛋白结合口服碘水造模共 7 周。 造模成功大鼠随机分为模型组 10 只、给药组 10 只。 甲炎康泰颗粒剂连续干预 8 周后,ELISA 法检测甲状腺抗体水平;HE 染色观察甲状腺病理改变;qPCR 法检测靶点因子基因 表达;抗体芯片法检测靶点蛋白表达并绘制聚类热图。 结果 模型组 TPOAb、TGAb 较正常组显著升高,给药组 TPOAb、TGAb 较模型组显著降低。 模型组甲状腺滤泡细胞破坏、大量淋巴细胞浸润,给药组甲状腺病理改变较模型组缓解。 模型组 IFN-γ、TNF-α、IL-17、CCL3、CX3CL1 基因表达量较正常组升高,给药组 IL-4、IFN-γ、TNF-α、IL- 17、CCL3、CX3CL1 较模型组降低。 各组间细胞因子聚类较好,模型组 IL-17、CCL3、CX3CL1 蛋白表达量较正常组升 高,给药组 IL-4、IFN-γ、TNF-α、CCL3、CX3CL1 较模型组降低。 结论 甲炎康泰颗粒剂可调节免疫相关细胞因子而减轻自身免疫性甲状腺炎大鼠甲状腺损伤。
Objective This study aimed to investigate the effects of JiaYanKangTai granules on immune- associated cytokines in rats with autoimmune thyroiditis, and the mechanisms for treating autoimmune thyroiditis. Methods 30 Lewis rats were randomly assigned into the control group (CON, n= 10) and the induced group (n= 20). The induced rats were then randomly divided into the model group (EAT, n= 10) and the administrative group ( JYKT, n= 10) after immunization by subcutaneous injections of thyroglobulin and drinking iodine-containing water for 7 weeks. Rats of the JYKT group were administrated JiaYanKangTai granules for 8 weeks. The expression levels of anti-thyroperoxidase (TPOAb) and anti-thyroglobulin ( TGAb) were detected. Pathological changes of the thyroid were observed. Gene and protein expression levels of multiple cytokines were detected by qPCR and antibody arrays, respectively. Differentially- expressed cytokines were analyzed and cluster heatmapping was performed. Results Expression levels of TPOAb and TGAb were significantly higher in the EAT group than those in the CON group. Pathological changes to the thyroid were alleviated in the JYKT group compared with those in the EAT group. Gene expression levels of chemokine C-C Motif Chemokine Ligand 3 (CCL3), C-X3-C motif chemokine ligand 1 (CX3CL1), interleukin-17 (IL-17), interferon-γ (IFN- γ) and tumor necrosis factor-α (TNF-α) were significantly higher in the EAT group than those in the CON group. Gene expression levels of CCL3, CX3CL1, IL-4, IL-17, IFN-γ and TNF-α were significantly lower in the JYKT group than those in the EAT group. Differential-expressed cytokines were well-clustered. Protein expression levels of CCL3, CX3CL1 and IL- 17 were significantly higher in the EAT group than those in the CON group. Protein expression levels of CCL3, CX3CL1, IL-4, IFN-γ and TNF-α were significantly lower in the JYKT group than those in the EAT group. Conclusions JiaYanKangTai granules alleviated damage of the thyroid in autoimmune thyroiditis by regulating immune-related cytokines.