1. 西安交通大学医学部实验动物中心,西安 710061; 2. 天津医科大学第二医院血管外科,天津 300211
1.Laboratory Animal Center, Xi’an Jiaotong University School of Medicine, Xi’an 710061, China. 2. Department of Vascular Surgery, the Second Hospital of Tianjin Medical University, Tianjin 300211
目的 分析正常饮食情况下,术前体重对猪胰弹性蛋白酶(PPE)诱导的小鼠腹主动脉瘤进展的影响。 方法 回顾分析本实验室最近半年 44 例 PPE 诱导的小鼠腹主动脉瘤模型中,术前不同体重对后期腹主动脉 直径和腹主动脉瘤进展的影响,分析两者的相关性。对术前体重低于 25 g 的 8 例入选小鼠(模型组 1)和术前体重高于 30 g 的 8 例入选小鼠(模型组 2)进行体重、脂肪分布、腹主动脉直径、腹主动脉直径变化、腹主动脉瘤病变组织学和免疫组织化学比较。 正常阴性对照小鼠未给与任何处理。 结果 44 例小鼠腹主动脉瘤血管直径和术前体 重的相关系数为 0. 005,未发现体重对腹主动脉瘤进展有显著影响。进一步分析发现,模型 1 组和模型 2 组在术后 14 d 血管直径(1. 35 mm vs 1. 27 mm)和血管直径变化上(0. 85 mm vs 0. 72 mm)差异没有显著性。 相比正常血管, 模型组 1 和模型组 2 小鼠在术后 14 d,腹主动脉肾下灌注段直径显著增加,全部形成动脉瘤。 组织学呈弹性纤维 断裂、平滑肌细胞耗竭、炎症反应增加和血管新生异常等典型的腹主动脉瘤病变特征。 结论 在正常饮食小鼠,体重未见影响腹主动脉瘤的进展。
Objective This study aimed to analyze the effect of preoperative body weight on the progression of porcine pancreatic elastase (PPE)-induced abdominal aortic aneurysm (AAA) in mice fed a normal diet. Methods We retrospectively analyzed the association of preoperative body weight and postoperative abdominal aortic diameter in 44 PPE- induced abdominal aortic aneurysm mouse models in our laboratory. Sixteen mice, divided into group 1 (n= 8, preoperative weight less than 25 g) and group 2 (n= 8, preoperative weight of more than 30 g) were chosen for the following analysis. The body weight, fat distribution, abdominal aorta diameter and diameter changes of all mice were analyzed. Comparative analysis of histology and immunohistochemistry of AAA lesions was conducted among group 1, group 2 and normal control animals. The normal control mice were free of surgery. Results The correlation coefficient between the aortic diameter and preoperative body weight was 0. 005 (using the 44 included mice). No significant influence of body weight was found on the progression of AAA in mice fed a normal diet in this study. Further analysis found that there were no significant differences in the abdominal aortic diameter (1. 35 mm vs 1. 27 mm) and diameter changes (0. 85 mm vs 0. 72 mm) between the model groups 1 and 2 at day 14 after the surgery. Compared with normal control mice, PPE-treated mice in model groups 1 and 2 had a significant increase in the diameter of the infrarenal aorta at postoperative day 14, and all formed aneurysms. Histological observations showed typical AAA lesion features, such as elastic fiber breakage, smooth muscle exhaustion, increased inflammation and abnormal angiogenesis. Conclusions Body weight was not associated with the progression of PPE-induced AAA in mice fed a normal diet.