Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
目的 通过文献数据挖掘与实验研究相结合的方法,挖掘洛哌丁胺诱导便秘小鼠模型造模特点及影响因素,为建立稳定的实验便秘模型提供一定参考。 方法 文献研究部分以便秘及动物模型为主题词,检索中英文数据库,筛选使用洛哌丁胺诱导便秘小鼠模型的实验研究,提取资料并进行分析;动物实验部分,采用雄性 C57BL/ 6 小鼠,给予 5 mg / kg 及 10 mg / kg 的洛哌丁胺灌胃,每日给药 1 次。 从含水率、首次黑便时间、小肠推进率等评价造模效果,观察洛哌丁胺不同剂量及给药时间对便秘小鼠的影响。 结果 纳入符合标准的文献 69 篇,洛哌丁胺给药剂量多为 5、10 mg / kg,多用灌胃给药,频率为给药 1 次,给药时间多为 30 min。 根据文献研究结果,实验部分从造模第 1 天开始,5 mg / kg 及 10 mg / kg 组与空白组相比含水率显著降低,差异具有统计学意义(P< 0. 05), 而造模第 7 ~ 14 天,5 mg / kg 组与 10 mg / kg 相比含水率明显升高(P< 0. 05),对比造模 3、7、14 d 后含水率变化情况,各模型组均在停药 1 d 后升高,至停药后 14 d 含水率均保持在较高水平;对比造模 3、7、14 d 的首次黑便时间, 造模 7、14 d 的 5 mg / kg 及 10 mg / kg 组与空白组及造模 3 d 的 5 mg / kg 组相比均升高,差异具有统计学意义(P< 0. 05);对比小肠推进率,各组间差异无统计学意义(P> 0. 05)。 结论 涉及即时效应实验可选择予以小鼠洛哌丁胺灌胃 1 次进行便秘造模,其他长期干预实验可选择予以小鼠 10 mg / kg 的洛哌丁胺浓度灌胃,于造模第 3 天开始干预,同时继续灌胃洛哌丁胺维持造模,以维持模型稳定性。
Objective To explore influencing factors of loperamide-induced constipation in mouse models by combining literature data mining and experimental research, to provide a reference for the establishment of a stable experimental constipation model. Methods Literature study, Constipation and animal model were used to search main titles, and then relevant literature from Chinese and English databases were retrieved. Then experimental studies were screened for mouse models of constipation induced by loperamide. Finally, data were extracted and analyzed by researchers. Experimental study, Male C57BL/ 6 mice were given 5 and 10 mg / kg loperamide orally once daily. We evaluated water content, timing of first melena and the intestinal propulsion rate to observe the effects of different doses and administration time of loperamide on mice with constipation. Results Literature study, We included 69 articles that met the standards, among which it was found that loperamide was mostly administered at doses of 5 and 10 mg / kg, with multiple intragastric administrations, the frequency of administration was once, and time of administration was mostly 30 min. Experimental study: From the day 1 of modeling, the water content of the 5 and 10 mg / kg groups significantly decreased (P< 0. 05) compared with the blank group. From day 7 to 14 of modeling, the water content of the 5 mg / kg group was significantly increased compared with the 10 mg / kg group (P< 0. 05). Comparison of changes in water content at 3, 7 and 14 days after modeling showed that the water content in each model group increased 1 day after drug withdrawal, and remained at high levels at 14 days after drug withdrawal. The timing of the first melena between day 7 and 14 of modeling was significantly increased (P< 0. 05) in the 5 and 10 mg / kg groups compared with the blank group. There were no significant differences in small intestinal propulsion rates among all groups ( P> 0. 05). Conclusions In the immediate effect experiment, loperamide was administered to mice for one time constipation modeling. For long-term intervention experiments, we can choose to treat mice with 10 mg / kg loperamide on the third day of modeling, and continue administration of loperamide to maintain the stability of the model.